Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 236656..237335 | Replicon | chromosome |
| Accession | NC_010473 | ||
| Organism | Escherichia coli str. K-12 substr. DH10B | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | ECDH10B_RS01145 | Protein ID | WP_000854672.1 |
| Coordinates | 236656..236997 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | ECDH10B_RS01150 | Protein ID | WP_000070395.1 |
| Coordinates | 237018..237335 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECDH10B_RS01120 (ECDH10B_0222) | 231933..232334 | + | 402 | WP_000174689.1 | sigma factor-binding protein Crl | - |
| ECDH10B_RS01125 (ECDH10B_0223) | 232373..233428 | - | 1056 | WP_000749863.1 | phosphoporin PhoE | - |
| ECDH10B_RS01130 (ECDH10B_0224) | 233716..234819 | + | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| ECDH10B_RS01135 (ECDH10B_0225) | 234831..236084 | + | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| ECDH10B_RS01145 (ECDH10B_0227) | 236656..236997 | - | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| ECDH10B_RS01150 (ECDH10B_0228) | 237018..237335 | - | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| ECDH10B_RS23500 (ECDH10B_0229) | 237354..237575 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| ECDH10B_RS01155 (ECDH10B_0230) | 237584..238060 | - | 477 | WP_000811693.1 | RadC family protein | - |
| ECDH10B_RS01160 (ECDH10B_0231) | 238076..238534 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| ECDH10B_RS01165 (ECDH10B_0232) | 238632..238871 | - | 240 | WP_000194654.1 | DUF905 domain-containing protein | - |
| ECDH10B_RS01170 (ECDH10B_0233) | 238948..239415 | - | 468 | WP_001547765.1 | hypothetical protein | - |
| ECDH10B_RS25845 (ECDH10B_0234) | 239438..239881 | - | 444 | WP_000824223.1 | hypothetical protein | - |
| ECDH10B_RS25850 (ECDH10B_0235) | 239881..240108 | - | 228 | WP_001548158.1 | hypothetical protein | - |
| ECDH10B_RS25855 (ECDH10B_0236) | 240104..240295 | - | 192 | Protein_226 | DeoR family transcriptional regulator | - |
| ECDH10B_RS01185 (ECDH10B_0237) | 240512..241333 | - | 822 | WP_000197389.1 | DUF945 domain-containing protein | - |
| ECDH10B_RS01190 (ECDH10B_0238) | 241425..242288 | - | 864 | WP_001065553.1 | GTPase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T22678 WP_000854672.1 NC_010473:c236997-236656 [Escherichia coli str. K-12 substr. DH10B]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T22678 NC_010473:c236997-236656 [Escherichia coli str. K-12 substr. DH10B]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT22678 WP_000070395.1 NC_010473:c237335-237018 [Escherichia coli str. K-12 substr. DH10B]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT22678 NC_010473:c237335-237018 [Escherichia coli str. K-12 substr. DH10B]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|