Detailed information of TA system TA226764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 308863..309449
• Replicon: chromosome
• Accession: NZ_CP088136
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain SCPM-O-B-4515

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A3V9X0E4
• Locus tag: LRM91_RS01415
• Protein ID: WP_000174964.1
• Coordinates: 309081..309449 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: V7IU48
• Locus tag: LRM91_RS01410
• Protein ID: WP_001522145.1
• Coordinates: 308863..309084 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM91_RS01385 (303884)	303884..304993	+	1110	WP_000822976.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LRM91_RS01390 (305053)	305053..305979	+	927	WP_000003007.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LRM91_RS01395 (305976)	305976..307253	+	1278	WP_000803764.1	branched chain amino acid ABC transporter permease LivM	-
LRM91_RS01400 (307250)	307250..308017	+	768	WP_249895384.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LRM91_RS01405 (308019)	308019..308732	+	714	WP_000416114.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LRM91_RS01410 (308863)	308863..309084	+	222	WP_001522145.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LRM91_RS01415 (309081)	309081..309449	+	369	WP_000174964.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LRM91_RS01420 (309708)	309708..311024	+	1317	WP_000624755.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LRM91_RS01425 (311129)	311129..312016	+	888	WP_249895385.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LRM91_RS01430 (312013)	312013..312858	+	846	WP_000572197.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LRM91_RS01435 (312860)	312860..313930	+	1071	WP_000907835.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	307250..314667	7417

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-114)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.91 Da
• Isoelectric Point: 6.7252

>T226764 WP_000174964.1 NZ_CP088136:309081-309449 [Salmonella enterica subsp. enterica serovar Typhimurium]
MTLQLISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLAANPDFVDMTVDAAAGRLTLEQIVVRLRA

Nucleotide

• Length: 369 bp

>T226764 NZ_CP122622:c74186-73905 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAGATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8166.17 Da
• Isoelectric Point: 5.0775

>AT226764 WP_001522145.1 NZ_CP088136:308863-309084 [Salmonella enterica subsp. enterica serovar Typhimurium]
MRTVNYSEARQNLAEVLESAVTAGPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGNELRELADK

Nucleotide

• Length: 222 bp

>AT226764 NZ_CP122622:c74427-74176 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A3V9X0E4