Detailed information of TA system TA226762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 30284..30909
• Replicon: plasmid pB-4493_1
• Accession: NZ_CP088135
• Organism: Salmonella enterica subsp. enterica serovar Gallinarum strain SCPM-O-B-4493

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LRM90_RS23505
• Protein ID: WP_000911322.1
• Coordinates: 30511..30909 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8F2UQ48
• Locus tag: LRM90_RS23500
• Protein ID: WP_000450263.1
• Coordinates: 30284..30511 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM90_RS23470 (LRM90_23470)	25549..25718	-	170	Protein_34	phospholipase	-
LRM90_RS23475 (LRM90_23475)	25769..25966	-	198	WP_223155440.1	hypothetical protein	-
LRM90_RS23480 (LRM90_23480)	25940..26213	-	274	Protein_36	disulfide bond formation protein DsbA	-
LRM90_RS23485 (LRM90_23485)	26330..26893	-	564	WP_000139307.1	fertility inhibition protein FinO	-
LRM90_RS23490 (LRM90_23490)	26948..27688	-	741	WP_000177630.1	conjugal transfer pilus acetylase TraX	-
LRM90_RS23495 (LRM90_23495)	27708..30202	-	2495	Protein_39	MobF family relaxase	-
LRM90_RS23500 (LRM90_23500)	30284..30511	+	228	WP_000450263.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LRM90_RS23505 (LRM90_23505)	30511..30909	+	399	WP_000911322.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRM90_RS23510 (LRM90_23510)	30918..33116	-	2199	WP_079970305.1	type IV conjugative transfer system coupling protein TraD	-
LRM90_RS23515 (LRM90_23515)	33488..34219	-	732	WP_001541558.1	conjugal transfer complement resistance protein TraT	-
LRM90_RS23520 (LRM90_23520)	34522..34764	-	243	WP_024131413.1	hypothetical protein	-
LRM90_RS23525 (LRM90_23525)	34761..35462	-	702	WP_148201696.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	spvB / spvC / pefB / pefA / pefC / pefD	1..49556	49556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14887.13 Da
• Isoelectric Point: 8.5264

>T226762 WP_000911322.1 NZ_CP088135:30511-30909 [Salmonella enterica subsp. enterica serovar Gallinarum]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLDVLDYDTPAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVGGLRTEDWS

Nucleotide

• Length: 399 bp

>T226762 NZ_CP122622:270-548 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCAAGCCAGGACAGAAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8532.53 Da
• Isoelectric Point: 4.7513

>AT226762 WP_000450263.1 NZ_CP088135:30284-30511 [Salmonella enterica subsp. enterica serovar Gallinarum]
METSVFLSNRSQAVRLPKAVALPEHVKRVEVIAVGRTRIITPAGESWDEWFDGNNVSADFMDNREQPSVQERESF

Nucleotide

• Length: 228 bp

>AT226762 NZ_CP122622:1-270 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCGGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCTCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA