Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4281386..4281883 Replicon chromosome
Accession NC_010468
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECOLC_RS20965 Protein ID WP_001173343.1
Coordinates 4281386..4281559 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECOLC_RS20970 Protein ID WP_000398619.1
Coordinates 4281587..4281883 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOLC_RS20955 4278056..4279513 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
ECOLC_RS20960 4279750..4281267 + 1518 WP_001295087.1 lysine--tRNA ligase -
ECOLC_RS20965 4281386..4281559 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECOLC_RS20970 4281587..4281883 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECOLC_RS20975 4282110..4282382 - 273 WP_000405647.1 N-acetyltransferase -
ECOLC_RS20980 4282394..4282624 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
ECOLC_RS20985 4282805..4284436 + 1632 WP_001216477.1 sensor histidine kinase -
ECOLC_RS20990 4284433..4285152 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4265907..4281883 15976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T22675 WP_001173343.1 NC_010468:c4281559-4281386 [Escherichia coli ATCC 8739]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T22675 NC_010468:c4281559-4281386 [Escherichia coli ATCC 8739]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT22675 WP_000398619.1 NC_010468:c4281883-4281587 [Escherichia coli ATCC 8739]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT22675 NC_010468:c4281883-4281587 [Escherichia coli ATCC 8739]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References