Detailed information of TA system TA226749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2460794..2461380
• Replicon: chromosome
• Accession: NZ_CP088134
• Organism: Salmonella enterica subsp. enterica serovar Gallinarum strain SCPM-O-B-4493

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q57IR9
• Locus tag: LRM90_RS11865
• Protein ID: WP_000174963.1
• Coordinates: 2461012..2461380 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: M7SDJ3
• Locus tag: LRM90_RS11860
• Protein ID: WP_001520924.1
• Coordinates: 2460794..2461015 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM90_RS11835 (2455821)	2455821..2456924	+	1104	WP_023235350.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LRM90_RS11840 (2456984)	2456984..2457910	+	927	WP_000003005.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LRM90_RS11845 (2457907)	2457907..2459184	+	1278	WP_001541045.1	branched chain amino acid ABC transporter permease LivM	-
LRM90_RS11850 (2459181)	2459181..2459948	+	768	WP_000082080.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LRM90_RS11855 (2459950)	2459950..2460663	+	714	WP_000416114.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LRM90_RS11860 (2460794)	2460794..2461015	+	222	WP_001520924.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LRM90_RS11865 (2461012)	2461012..2461380	+	369	WP_000174963.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LRM90_RS11870 (2461639)	2461639..2462955	+	1317	WP_000624747.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LRM90_RS11875 (2463060)	2463060..2463947	+	888	WP_038394052.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LRM90_RS11880 (2463944)	2463944..2464789	+	846	WP_023235349.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LRM90_RS11885 (2464792)	2464792..2465862	+	1071	WP_011264401.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2456984..2466599	9615

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13573.85 Da
• Isoelectric Point: 6.7252

>T226749 WP_000174963.1 NZ_CP088134:2461012-2461380 [Salmonella enterica subsp. enterica serovar Gallinarum]
MTLQLISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLAANPDFVDMTVDAAAGRLTLEQIAVRLRA

Nucleotide

• Length: 369 bp

>T226749 NZ_CP122620:c2075830-2075636 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGGCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCGCTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8152.14 Da
• Isoelectric Point: 5.0775

>AT226749 WP_001520924.1 NZ_CP088134:2460794-2461015 [Salmonella enterica subsp. enterica serovar Gallinarum]
MRTVNYSEARQNLAEVLESAVTGGPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGNELRELADK

Nucleotide

• Length: 222 bp

>AT226749 NZ_CP122620:2075459-2075637 [Escherichia coli]
GAGGGCAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCCGA
GAGCATTTTTTCGCATTCCGATTTGGTTAACTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A5H6KCD5

Antitoxin

• AlphaFold DB: A0A6C6Z871