Detailed information of TA system TA226739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 50679..51204
• Replicon: plasmid pEC81-mcr10
• Accession: NZ_CP088133
• Organism: Escherichia coli strain 81

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: LRM32_RS24265
• Protein ID: WP_013023785.1
• Coordinates: 50899..51204 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: LRM32_RS24260
• Protein ID: WP_001568025.1
• Coordinates: 50679..50897 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM32_RS24230 (LRM32_24230)	46458..46874	-	417	WP_000754567.1	type II toxin-antitoxin system VapC family toxin	-
LRM32_RS24235 (LRM32_24235)	46871..47101	-	231	WP_023293777.1	type II toxin-antitoxin system VapB family antitoxin	-
LRM32_RS24240 (LRM32_24240)	47377..47877	+	501	WP_021312477.1	HEPN family nuclease	-
LRM32_RS24245 (LRM32_24245)	47890..48666	+	777	WP_021312478.1	hypothetical protein	-
LRM32_RS24250 (LRM32_24250)	48828..49076	+	249	WP_023332841.1	hypothetical protein	-
LRM32_RS24255 (LRM32_24255)	49130..50116	-	987	WP_021312480.1	hypothetical protein	-
LRM32_RS24260 (LRM32_24260)	50679..50897	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LRM32_RS24265 (LRM32_24265)	50899..51204	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
LRM32_RS24270 (LRM32_24270)	51391..52395	+	1005	WP_001568027.1	hypothetical protein	-
LRM32_RS24275 (LRM32_24275)	52579..53358	+	780	WP_001568028.1	site-specific integrase	-
LRM32_RS24280 (LRM32_24280)	53416..53673	-	258	WP_000764642.1	hypothetical protein	-
LRM32_RS24285 (LRM32_24285)	53802..53915	-	114	WP_014343462.1	hypothetical protein	-
LRM32_RS24290 (LRM32_24290)	54546..55301	+	756	WP_001568031.1	replication initiation protein RepE	-
LRM32_RS24295 (LRM32_24295)	55591..56025	-	435	WP_021312407.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-10	mrkA / mrkB / mrkC / mrkF / mrkJ	1..62662	62662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T226739 WP_013023785.1 NZ_CP088133:50899-51204 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T226739 NZ_CP122619:24252-24530 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT226739 WP_001568025.1 NZ_CP088133:50679-50897 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT226739 NZ_CP122619:23983-24252 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8