Detailed information of TA system TA226738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 46458..47101
• Replicon: plasmid pEC81-mcr10
• Accession: NZ_CP088133
• Organism: Escherichia coli strain 81

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A155IU92
• Locus tag: LRM32_RS24230
• Protein ID: WP_000754567.1
• Coordinates: 46458..46874 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A155IUD1
• Locus tag: LRM32_RS24235
• Protein ID: WP_023293777.1
• Coordinates: 46871..47101 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM32_RS24195 (LRM32_24195)	41533..42114	+	582	WP_023304048.1	TetR/AcrR family transcriptional regulator	-
LRM32_RS24200 (LRM32_24200)	42119..42457	+	339	WP_000888080.1	carboxymuconolactone decarboxylase family protein	-
LRM32_RS24205 (LRM32_24205)	42487..42816	-	330	WP_008502229.1	thioredoxin family protein	-
LRM32_RS24210 (LRM32_24210)	43029..44135	+	1107	WP_006687059.1	alkene reductase	-
LRM32_RS24215 (LRM32_24215)	44201..44902	+	702	WP_008502228.1	DsbA family oxidoreductase	-
LRM32_RS24220 (LRM32_24220)	44968..45105	+	138	Protein_50	sugar dehydrogenase	-
LRM32_RS24225 (LRM32_24225)	45139..46107	+	969	WP_008502226.1	IS5-like element IS903B family transposase	-
LRM32_RS24230 (LRM32_24230)	46458..46874	-	417	WP_000754567.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRM32_RS24235 (LRM32_24235)	46871..47101	-	231	WP_023293777.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LRM32_RS24240 (LRM32_24240)	47377..47877	+	501	WP_021312477.1	HEPN family nuclease	-
LRM32_RS24245 (LRM32_24245)	47890..48666	+	777	WP_021312478.1	hypothetical protein	-
LRM32_RS24250 (LRM32_24250)	48828..49076	+	249	WP_023332841.1	hypothetical protein	-
LRM32_RS24255 (LRM32_24255)	49130..50116	-	987	WP_021312480.1	hypothetical protein	-
LRM32_RS24260 (LRM32_24260)	50679..50897	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
LRM32_RS24265 (LRM32_24265)	50899..51204	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-10	mrkA / mrkB / mrkC / mrkF / mrkJ	1..62662	62662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15108.61 Da
• Isoelectric Point: 8.5403

>T226738 WP_000754567.1 NZ_CP088133:c46874-46458 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T226738 NZ_CP122618:9477-9728 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8706.73 Da
• Isoelectric Point: 4.4299

>AT226738 WP_023293777.1 NZ_CP088133:c47101-46871 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Nucleotide

• Length: 231 bp

>AT226738 NZ_CP122618:9715-9942 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Structures

Toxin

• AlphaFold DB: A0A155IU92

Antitoxin

• AlphaFold DB: A0A155IUD1