Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3820871..3821658 Replicon chromosome
Accession NZ_CP088131
Organism Escherichia coli strain 81

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2H9G3C1
Locus tag LRM32_RS18775 Protein ID WP_001568519.1
Coordinates 3821281..3821658 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2H9G3E7
Locus tag LRM32_RS18770 Protein ID WP_000066236.1
Coordinates 3820871..3821230 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM32_RS18730 (3816567) 3816567..3816713 + 147 WP_023563913.1 hypothetical protein -
LRM32_RS18735 (3816726) 3816726..3816941 + 216 WP_041036768.1 hypothetical protein -
LRM32_RS18740 (3817055) 3817055..3817465 + 411 WP_042851426.1 hypothetical protein -
LRM32_RS18745 (3817639) 3817639..3818469 + 831 WP_225858599.1 hypothetical protein -
LRM32_RS18750 (3818558) 3818558..3819376 + 819 WP_016236913.1 DUF932 domain-containing protein -
LRM32_RS18755 (3819644) 3819644..3820114 + 471 WP_241466651.1 antirestriction protein -
LRM32_RS18760 (3820126) 3820126..3820605 + 480 WP_000437750.1 DNA repair protein RadC -
LRM32_RS18765 (3820626) 3820626..3820847 + 222 WP_000691981.1 DUF987 domain-containing protein -
LRM32_RS18770 (3820871) 3820871..3821230 + 360 WP_000066236.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LRM32_RS18775 (3821281) 3821281..3821658 + 378 WP_001568519.1 TA system toxin CbtA family protein Toxin
LRM32_RS18780 (3821655) 3821655..3822146 + 492 WP_000777682.1 DUF5983 family protein -
LRM32_RS18785 (3822178) 3822178..3822381 + 204 WP_000413747.1 DUF957 domain-containing protein -
LRM32_RS18790 (3822462) 3822462..3823307 + 846 WP_016236912.1 DUF4942 domain-containing protein -
LRM32_RS18800 (3823649) 3823649..3824380 - 732 WP_001300756.1 DNA polymerase III subunit epsilon -
LRM32_RS18805 (3824445) 3824445..3824912 + 468 WP_000917883.1 ribonuclease HI -
LRM32_RS18810 (3824909) 3824909..3825631 - 723 WP_001295200.1 class I SAM-dependent methyltransferase -
LRM32_RS18815 (3825665) 3825665..3826420 + 756 WP_001052721.1 hydroxyacylglutathione hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3800691..3826420 25729


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14211.00 Da        Isoelectric Point: 7.4209

>T226737 WP_001568519.1 NZ_CP088131:3821281-3821658 [Escherichia coli]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKTTGLMTRNDYRTVTDITTGKYRGGHR

Download         Length: 378 bp

>T226737 NZ_CP122617:c4663492-4663181 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13127.02 Da        Isoelectric Point: 7.0261

>AT226737 WP_000066236.1 NZ_CP088131:3820871-3821230 [Escherichia coli]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Download         Length: 360 bp

>AT226737 NZ_CP122617:c4663180-4662890 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H9G3C1

Antitoxin

Source ID Structure
AlphaFold DB A0A2H9G3E7

References