Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3511010..3511628 Replicon chromosome
Accession NZ_CP088131
Organism Escherichia coli strain 81

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LRM32_RS17185 Protein ID WP_001291435.1
Coordinates 3511410..3511628 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LRM32_RS17180 Protein ID WP_000344800.1
Coordinates 3511010..3511384 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM32_RS17170 (3506099) 3506099..3507292 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LRM32_RS17175 (3507315) 3507315..3510464 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LRM32_RS17180 (3511010) 3511010..3511384 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LRM32_RS17185 (3511410) 3511410..3511628 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LRM32_RS17190 (3511800) 3511800..3512351 + 552 WP_000102564.1 maltose O-acetyltransferase -
LRM32_RS17195 (3512467) 3512467..3512937 + 471 WP_000136192.1 YlaC family protein -
LRM32_RS17200 (3513101) 3513101..3514651 + 1551 WP_016241944.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LRM32_RS17205 (3514693) 3514693..3515046 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LRM32_RS17215 (3515425) 3515425..3515736 + 312 WP_000409911.1 MGMT family protein -
LRM32_RS17220 (3515767) 3515767..3516339 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T226733 WP_001291435.1 NZ_CP088131:3511410-3511628 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T226733 NZ_CP122617:3751754-3752032 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT226733 WP_000344800.1 NZ_CP088131:3511010-3511384 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT226733 NZ_CP122617:3751491-3751751 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References