Detailed information of TA system TA226732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3116098..3116803
• Replicon: chromosome
• Accession: NZ_CP088131
• Organism: Escherichia coli strain 81

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LRM32_RS15235
• Protein ID: WP_000539521.1
• Coordinates: 3116098..3116484 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LRM32_RS15240
• Protein ID: WP_001280945.1
• Coordinates: 3116474..3116803 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM32_RS15215 (3112102)	3112102..3112728	+	627	WP_016241992.1	glutathione S-transferase GstB	-
LRM32_RS15220 (3112725)	3112725..3113840	-	1116	WP_000555031.1	aldose sugar dehydrogenase YliI	-
LRM32_RS15225 (3113951)	3113951..3114334	-	384	WP_000497137.1	biofilm formation regulator BssR	-
LRM32_RS15230 (3114547)	3114547..3115872	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LRM32_RS15235 (3116098)	3116098..3116484	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRM32_RS15240 (3116474)	3116474..3116803	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LRM32_RS15245 (3116873)	3116873..3118201	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
LRM32_RS15250 (3118209)	3118209..3119786	-	1578	Protein_2995	cyclic diguanylate phosphodiesterase	-
LRM32_RS15255 (3119841)	3119841..3120538	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
LRM32_RS15260 (3120553)	3120553..3121299	-	747	Protein_2997	CHASE9 sensor domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T226732 WP_000539521.1 NZ_CP088131:3116098-3116484 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T226732 NZ_CP122617:3566368-3566910 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT226732 WP_001280945.1 NZ_CP088131:3116474-3116803 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT226732 NZ_CP122617:3566073-3566384 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6