Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2533676..2534314 Replicon chromosome
Accession NZ_CP088131
Organism Escherichia coli strain 81

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag LRM32_RS12430 Protein ID WP_000813794.1
Coordinates 2534138..2534314 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LRM32_RS12425 Protein ID WP_001270286.1
Coordinates 2533676..2534092 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM32_RS12405 (2528828) 2528828..2529769 - 942 WP_001251313.1 ABC transporter permease -
LRM32_RS12410 (2529770) 2529770..2530783 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
LRM32_RS12415 (2530801) 2530801..2531946 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
LRM32_RS12420 (2532191) 2532191..2533597 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
LRM32_RS12425 (2533676) 2533676..2534092 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LRM32_RS12430 (2534138) 2534138..2534314 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LRM32_RS12435 (2534536) 2534536..2534766 + 231 WP_000494244.1 YncJ family protein -
LRM32_RS12440 (2534858) 2534858..2536819 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LRM32_RS12445 (2536892) 2536892..2537428 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
LRM32_RS12450 (2537520) 2537520..2538695 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T226731 WP_000813794.1 NZ_CP088131:c2534314-2534138 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T226731 NZ_CP122617:3532396-3532614 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT226731 WP_001270286.1 NZ_CP088131:c2534092-2533676 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT226731 NZ_CP122617:3531996-3532370 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References