Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 923229..923883 Replicon chromosome
Accession NZ_CP088131
Organism Escherichia coli strain 81

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LRM32_RS04585 Protein ID WP_000244777.1
Coordinates 923476..923883 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LRM32_RS04580 Protein ID WP_000354046.1
Coordinates 923229..923495 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM32_RS04555 (918398) 918398..919141 + 744 WP_000951964.1 SDR family oxidoreductase -
LRM32_RS04560 (919198) 919198..920631 - 1434 WP_241466666.1 6-phospho-beta-glucosidase BglA -
LRM32_RS04565 (920676) 920676..920987 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LRM32_RS04570 (921151) 921151..921810 + 660 WP_000250274.1 hemolysin III family protein -
LRM32_RS04575 (922006) 922006..922986 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
LRM32_RS04580 (923229) 923229..923495 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LRM32_RS04585 (923476) 923476..923883 + 408 WP_000244777.1 protein YgfX Toxin
LRM32_RS04590 (923923) 923923..924444 - 522 WP_001055874.1 flavodoxin FldB -
LRM32_RS04595 (924556) 924556..925452 + 897 WP_000806628.1 site-specific tyrosine recombinase XerD -
LRM32_RS04600 (925477) 925477..926187 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LRM32_RS04605 (926193) 926193..927926 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T226720 WP_000244777.1 NZ_CP088131:923476-923883 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T226720 NZ_CP122617:994744-995079 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGTTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT226720 WP_000354046.1 NZ_CP088131:923229-923495 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT226720 NZ_CP122617:994496-994744 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References