Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 26482..27007 Replicon plasmid pKP57-mcr8
Accession NZ_CP088130
Organism Klebsiella pneumoniae strain 57

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag LRM34_RS27625 Protein ID WP_025714517.1
Coordinates 26702..27007 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag LRM34_RS27620 Protein ID WP_006788213.1
Coordinates 26482..26700 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM34_RS27600 (LRM34_27600) 21729..22697 + 969 WP_241478672.1 IS5-like element IS903B family transposase -
LRM34_RS27605 (LRM34_27605) 23169..23960 - 792 WP_221928726.1 ribbon-helix-helix domain-containing protein -
LRM34_RS27610 (LRM34_27610) 24143..25171 - 1029 WP_032445668.1 Abi family protein -
LRM34_RS27615 (LRM34_27615) 25332..25913 - 582 WP_072310991.1 hypothetical protein -
LRM34_RS27620 (LRM34_27620) 26482..26700 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LRM34_RS27625 (LRM34_27625) 26702..27007 + 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
LRM34_RS27630 (LRM34_27630) 27176..27571 + 396 WP_017899885.1 hypothetical protein -
LRM34_RS27635 (LRM34_27635) 27598..27912 + 315 WP_053389906.1 hypothetical protein -
LRM34_RS27640 (LRM34_27640) 28207..28923 + 717 Protein_31 hypothetical protein -
LRM34_RS27645 (LRM34_27645) 29121..29906 + 786 WP_046664219.1 site-specific integrase -
LRM34_RS27650 (LRM34_27650) 30355..31110 + 756 WP_001568031.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-8 - 1..100928 100928
- flank IS/Tn mcr-8 - 15672..22697 7025


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T226716 WP_025714517.1 NZ_CP088130:26702-27007 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T226716 NZ_CP122617:264982-265212 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT226716 WP_006788213.1 NZ_CP088130:26482-26700 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT226716 NZ_CP122617:265209-265568 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References