Detailed information of TA system TA226715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 60105..60841
• Replicon: plasmid pKP57-mcr10
• Accession: NZ_CP088129
• Organism: Klebsiella pneumoniae strain 57

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: LRM34_RS26885
• Protein ID: WP_003026803.1
• Coordinates: 60359..60841 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LRM34_RS26880
• Protein ID: WP_003026799.1
• Coordinates: 60105..60371 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM34_RS26850 (LRM34_26850)	55756..57156	+	1401	WP_004152084.1	copper resistance membrane spanning protein PcoS	-
LRM34_RS26855 (LRM34_26855)	57372..57806	+	435	WP_004118347.1	hypothetical protein	-
LRM34_RS26860 (LRM34_26860)	57986..58954	+	969	WP_072202995.1	IS5 family transposase	-
LRM34_RS26865 (LRM34_26865)	59012..59152	+	141	Protein_63	DNA-binding protein	-
LRM34_RS26870 (LRM34_26870)	59213..59416	+	204	WP_004206661.1	HHA domain-containing protein	-
LRM34_RS26875 (LRM34_26875)	59430..59660	+	231	WP_004206660.1	hypothetical protein	-
LRM34_RS26880 (LRM34_26880)	60105..60371	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LRM34_RS26885 (LRM34_26885)	60359..60841	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
LRM34_RS26890 (LRM34_26890)	61042..62445	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
LRM34_RS26895 (LRM34_26895)	62474..63106	-	633	WP_001567369.1	hypothetical protein	-
LRM34_RS26900 (LRM34_26900)	63332..64677	+	1346	Protein_70	ISNCY family transposase	-
LRM34_RS26905 (LRM34_26905)	64726..65121	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-10 / blaTEM-1B / floR / tet(A) / qnrB52 / sul1	mrkA / mrkB / mrkC / mrkC / mrkC / mrkD / mrkD / mrkF / mrkJ	1..186040	186040
	-	inside	IScluster/Tn	-	-	58031..66707	8676

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T226715 WP_003026803.1 NZ_CP088129:60359-60841 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T226715 NZ_CP122608:c22417-22157 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT226715 WP_003026799.1 NZ_CP088129:60105-60371 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT226715 NZ_CP122608:c22167-21916 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66