Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 31103..31746 Replicon plasmid pKP57-mcr10
Accession NZ_CP088129
Organism Klebsiella pneumoniae strain 57

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LRM34_RS26740 Protein ID WP_001044770.1
Coordinates 31330..31746 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LRM34_RS26735 Protein ID WP_001261282.1
Coordinates 31103..31333 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM34_RS26700 (LRM34_26700) 27223..27858 + 636 WP_009309912.1 type II CAAX endopeptidase family protein -
LRM34_RS26705 (LRM34_26705) 27966..28310 - 345 WP_009309911.1 hypothetical protein -
LRM34_RS26710 (LRM34_26710) 28632..29162 + 531 WP_223174905.1 hypothetical protein -
LRM34_RS26715 (LRM34_26715) 29131..29568 + 438 WP_009309910.1 hypothetical protein -
LRM34_RS26720 (LRM34_26720) 29759..30199 - 441 WP_047057603.1 hypothetical protein -
LRM34_RS26725 (LRM34_26725) 30218..30841 - 624 WP_223345396.1 hypothetical protein -
LRM34_RS26730 (LRM34_26730) 30910..31146 - 237 Protein_36 hypothetical protein -
LRM34_RS26735 (LRM34_26735) 31103..31333 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LRM34_RS26740 (LRM34_26740) 31330..31746 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LRM34_RS26745 (LRM34_26745) 31820..33382 + 1563 WP_241462234.1 AAA family ATPase -
LRM34_RS26750 (LRM34_26750) 33367..34389 + 1023 WP_000361404.1 helicase UvrD -
LRM34_RS26755 (LRM34_26755) 34933..35841 + 909 WP_032425603.1 HNH endonuclease -
LRM34_RS26760 (LRM34_26760) 36027..36377 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-10 / blaTEM-1B / floR / tet(A) / qnrB52 / sul1 mrkA / mrkB / mrkC / mrkC / mrkC / mrkD / mrkD / mrkF / mrkJ 1..186040 186040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T226714 WP_001044770.1 NZ_CP088129:31330-31746 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T226714 NZ_CP122607:13497-13775 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGACGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT226714 WP_001261282.1 NZ_CP088129:31103-31333 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT226714 NZ_CP122607:13228-13497 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCACGGCGATGTGCTTCCTCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References