Detailed information of TA system TA226713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 20498..21023
• Replicon: plasmid pKP57-mcr10
• Accession: NZ_CP088129
• Organism: Klebsiella pneumoniae strain 57

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: LRM34_RS26665
• Protein ID: WP_004197633.1
• Coordinates: 20498..20803 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A1D8K3R4
• Locus tag: LRM34_RS26670
• Protein ID: WP_006788213.1
• Coordinates: 20805..21023 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM34_RS26630 (LRM34_26630)	16079..17059	-	981	WP_014386491.1	IS5-like element ISKpn26 family transposase	-
LRM34_RS26635 (LRM34_26635)	17162..17275	+	114	WP_014343462.1	hypothetical protein	-
LRM34_RS26640 (LRM34_26640)	17404..17661	+	258	WP_009310077.1	hypothetical protein	-
LRM34_RS26645 (LRM34_26645)	17719..18497	-	779	Protein_19	site-specific integrase	-
LRM34_RS26650 (LRM34_26650)	18695..19451	-	757	Protein_20	hypothetical protein	-
LRM34_RS26655 (LRM34_26655)	19745..20080	-	336	WP_009309920.1	hypothetical protein	-
LRM34_RS26660 (LRM34_26660)	20129..20440	-	312	WP_009309919.1	hypothetical protein	-
LRM34_RS26665 (LRM34_26665)	20498..20803	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
LRM34_RS26670 (LRM34_26670)	20805..21023	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LRM34_RS26675 (LRM34_26675)	21625..21854	+	230	Protein_25	type II toxin-antitoxin system VapB family antitoxin	-
LRM34_RS26680 (LRM34_26680)	21851..22267	+	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	-
LRM34_RS26685 (LRM34_26685)	22308..23186	-	879	WP_032427228.1	restriction endonuclease	-
LRM34_RS26690 (LRM34_26690)	23848..25125	+	1278	WP_009309915.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-10 / blaTEM-1B / floR / tet(A) / qnrB52 / sul1	mrkA / mrkB / mrkC / mrkC / mrkC / mrkD / mrkD / mrkF / mrkJ	1..186040	186040
	-	flank	IS/Tn	-	-	16079..17059	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T226713 WP_004197633.1 NZ_CP088129:c20803-20498 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T226713 NZ_CP122604:c4935758-4935447 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7405

>AT226713 WP_006788213.1 NZ_CP088129:c21023-20805 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT226713 NZ_CP122604:c4935446-4935156 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5

Antitoxin

• AlphaFold DB: A0A1D8K3R4