Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9360..10003 Replicon plasmid pKP57-3
Accession NZ_CP088127
Organism Klebsiella pneumoniae strain 57

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag LRM34_RS25765 Protein ID WP_000754566.1
Coordinates 9587..10003 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LRM34_RS25760 Protein ID WP_001261276.1
Coordinates 9360..9590 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM34_RS25725 (LRM34_25725) 4479..4748 + 270 WP_000339857.1 hypothetical protein -
LRM34_RS25730 (LRM34_25730) 4925..5791 - 867 WP_004118283.1 replication initiation protein -
LRM34_RS25735 (LRM34_25735) 6321..6425 + 105 WP_032409716.1 hypothetical protein -
LRM34_RS25740 (LRM34_25740) 6554..6811 + 258 WP_000764642.1 hypothetical protein -
LRM34_RS25745 (LRM34_25745) 6869..7645 - 777 WP_000015958.1 site-specific integrase -
LRM34_RS25750 (LRM34_25750) 7642..8385 - 744 WP_000129823.1 hypothetical protein -
LRM34_RS25755 (LRM34_25755) 8436..8786 - 351 WP_000493378.1 hypothetical protein -
LRM34_RS25760 (LRM34_25760) 9360..9590 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LRM34_RS25765 (LRM34_25765) 9587..10003 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
LRM34_RS25770 (LRM34_25770) 10207..11063 + 857 Protein_13 IS3-like element ISEc15 family transposase -
LRM34_RS25775 (LRM34_25775) 11345..12421 + 1077 WP_000227969.1 IS110 family transposase -
LRM34_RS25780 (LRM34_25780) 13062..13145 + 84 Protein_15 SOS response-associated peptidase -
LRM34_RS25785 (LRM34_25785) 13203..14171 - 969 WP_241478672.1 IS5-like element IS903B family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / mph(A) / tet(A) - 1..83070 83070


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T226712 WP_000754566.1 NZ_CP088127:9587-10003 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T226712 NZ_CP122604:c4733821-4733435 [Escherichia coli]
GTGCTTGCCCCAACTGGGCCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCCGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATATCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT226712 WP_001261276.1 NZ_CP088127:9360-9590 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT226712 NZ_CP122604:c4734360-4733851 [Escherichia coli]
ATGGCATATCTGAAATTGATGAAGCTGCTCTATCTGTCTAACCGCAAGTCGATTTTGAAGCATGGCAGGATGATCGGCGA
AGATAGCCTTTACTCTATGAAATTTGGTCCAGTCATGTCGAATACGCTGAACTTGATTCGTGGTAAGGCTGAAGGTATTG
GTGACTACTGGTACAACCTGATAGAGACGAACGGGCATGATGTGTTGTTGCGTTCAGATCCGAGGGAAATGGATGCAGAC
GAGGTCTTTGATGAGTTGAGCCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCTGTATGGGCATATGAACCGATT
TGATCTCGCAAACATGACGCATTTAGAAAGCGTTTGTCCAGAGTGGCACAATCCTGGCAATTCTCGTAAGCCTATAGACC
TGAAAGAAATGCTGATCAGTGAGGGTAAAAGCGAGGATGAGGCTAATCGTATAATTGGCAAAATGGAAGAATCTCAGAAA
CTTAAGGAATTTTCTTTGCAATTATCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References