Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5175990..5176615 Replicon chromosome
Accession NZ_CP088126
Organism Klebsiella pneumoniae strain 57

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LRM34_RS25335 Protein ID WP_002882817.1
Coordinates 5175990..5176373 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LRM34_RS25340 Protein ID WP_004150355.1
Coordinates 5176373..5176615 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM34_RS25320 (LRM34_25320) 5173356..5174258 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LRM34_RS25325 (LRM34_25325) 5174255..5174890 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LRM34_RS25330 (LRM34_25330) 5174887..5175816 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LRM34_RS25335 (LRM34_25335) 5175990..5176373 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LRM34_RS25340 (LRM34_25340) 5176373..5176615 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LRM34_RS25345 (LRM34_25345) 5176820..5177737 + 918 WP_002882812.1 alpha/beta hydrolase -
LRM34_RS25350 (LRM34_25350) 5177751..5178692 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LRM34_RS25355 (LRM34_25355) 5178737..5179174 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LRM34_RS25360 (LRM34_25360) 5179171..5180031 - 861 WP_002882807.1 virulence factor BrkB family protein -
LRM34_RS25365 (LRM34_25365) 5180025..5180624 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T226711 WP_002882817.1 NZ_CP088126:c5176373-5175990 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T226711 NZ_CP122604:c4601544-4601371 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT226711 WP_004150355.1 NZ_CP088126:c5176615-5176373 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT226711 NZ_CP122604:c4601868-4601572 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References