Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3460852..3461470 Replicon chromosome
Accession NC_010468
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECOLC_RS16970 Protein ID WP_001291435.1
Coordinates 3461252..3461470 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECOLC_RS16965 Protein ID WP_000344800.1
Coordinates 3460852..3461226 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOLC_RS16955 3455941..3457134 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ECOLC_RS16960 3457157..3460306 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
ECOLC_RS16965 3460852..3461226 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECOLC_RS16970 3461252..3461470 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ECOLC_RS16975 3461642..3462193 + 552 WP_000102564.1 maltose O-acetyltransferase -
ECOLC_RS16980 3462309..3462779 + 471 WP_000136192.1 YlaC family protein -
ECOLC_RS16985 3462943..3464493 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECOLC_RS16990 3464535..3464888 - 354 WP_000878140.1 DUF1428 domain-containing protein -
ECOLC_RS16995 3465267..3465578 + 312 WP_000409911.1 MGMT family protein -
ECOLC_RS17000 3465609..3466181 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T22670 WP_001291435.1 NC_010468:3461252-3461470 [Escherichia coli ATCC 8739]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T22670 NC_010468:3461252-3461470 [Escherichia coli ATCC 8739]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT22670 WP_000344800.1 NC_010468:3460852-3461226 [Escherichia coli ATCC 8739]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT22670 NC_010468:3460852-3461226 [Escherichia coli ATCC 8739]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References