Detailed information of TA system TA226693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 26482..27007
• Replicon: plasmid pKP46-mcr8
• Accession: NZ_CP088125
• Organism: Klebsiella pneumoniae strain 46

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A6S4YF27
• Locus tag: LRM33_RS29090
• Protein ID: WP_025714517.1
• Coordinates: 26702..27007 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: LRM33_RS29085
• Protein ID: WP_001568025.1
• Coordinates: 26482..26700 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM33_RS29065 (LRM33_29065)	21729..22697	+	969	WP_075211999.1	IS5 family transposase	-
LRM33_RS29070 (LRM33_29070)	23169..23960	-	792	WP_221928726.1	ribbon-helix-helix domain-containing protein	-
LRM33_RS29075 (LRM33_29075)	24143..25171	-	1029	WP_032445668.1	Abi family protein	-
LRM33_RS29080 (LRM33_29080)	25332..25913	-	582	WP_072310991.1	hypothetical protein	-
LRM33_RS29085 (LRM33_29085)	26482..26700	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LRM33_RS29090 (LRM33_29090)	26702..27007	+	306	WP_025714517.1	type II toxin-antitoxin system toxin CcdB	Toxin
LRM33_RS29095 (LRM33_29095)	27176..27571	+	396	WP_017899885.1	hypothetical protein	-
LRM33_RS29100 (LRM33_29100)	27598..27912	+	315	WP_053389906.1	hypothetical protein	-
LRM33_RS29105 (LRM33_29105)	28207..28923	+	717	Protein_31	hypothetical protein	-
LRM33_RS29110 (LRM33_29110)	29121..29906	+	786	WP_046664219.1	site-specific integrase	-
LRM33_RS29115 (LRM33_29115)	30355..31110	+	756	WP_001568031.1	replication initiation protein RepE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-8	-	1..101184	101184
	-	flank	IS/Tn	mcr-8	-	15672..22697	7025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11562.25 Da
• Isoelectric Point: 5.6906

>T226693 WP_025714517.1 NZ_CP088125:26702-27007 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T226693 NZ_CP122604:c625442-624978 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATAGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCTTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCGATTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT226693 WP_001568025.1 NZ_CP088125:26482-26700 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT226693 NZ_CP122604:c625777-625442 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A6S4YF27

Antitoxin

• AlphaFold DB: A0A2J4ZZP8