Detailed information of TA system TA226692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 60117..60853
• Replicon: plasmid pKP46-mcr10
• Accession: NZ_CP088124
• Organism: Klebsiella pneumoniae strain 46

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: LRM33_RS28350
• Protein ID: WP_003026803.1
• Coordinates: 60371..60853 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LRM33_RS28345
• Protein ID: WP_003026799.1
• Coordinates: 60117..60383 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM33_RS28315 (LRM33_28315)	55768..57168	+	1401	WP_004152084.1	copper resistance membrane spanning protein PcoS	-
LRM33_RS28320 (LRM33_28320)	57384..57818	+	435	WP_004118347.1	hypothetical protein	-
LRM33_RS28325 (LRM33_28325)	57998..58966	+	969	WP_072202995.1	IS5 family transposase	-
LRM33_RS28330 (LRM33_28330)	59024..59164	+	141	Protein_63	DNA-binding protein	-
LRM33_RS28335 (LRM33_28335)	59225..59428	+	204	WP_004206661.1	HHA domain-containing protein	-
LRM33_RS28340 (LRM33_28340)	59442..59672	+	231	WP_004206660.1	hypothetical protein	-
LRM33_RS28345 (LRM33_28345)	60117..60383	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LRM33_RS28350 (LRM33_28350)	60371..60853	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
LRM33_RS28355 (LRM33_28355)	61054..62457	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
LRM33_RS28360 (LRM33_28360)	62486..63118	-	633	WP_001567369.1	hypothetical protein	-
LRM33_RS28365 (LRM33_28365)	63344..64689	+	1346	Protein_70	ISNCY family transposase	-
LRM33_RS28370 (LRM33_28370)	64738..65133	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-10 / blaTEM-1B / floR / tet(A) / qnrB52 / sul1	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..186056	186056
	-	inside	IScluster/Tn	-	-	58043..66719	8676

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T226692 WP_003026803.1 NZ_CP088124:60371-60853 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T226692 NZ_CP122603:13497-13775 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGACGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT226692 WP_003026799.1 NZ_CP088124:60117-60383 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT226692 NZ_CP122603:13228-13497 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCACGGCGATGTGCTTCCTCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66