Detailed information of TA system TA226691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 31115..31758
• Replicon: plasmid pKP46-mcr10
• Accession: NZ_CP088124
• Organism: Klebsiella pneumoniae strain 46

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: LRM33_RS28205
• Protein ID: WP_001044770.1
• Coordinates: 31342..31758 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: LRM33_RS28200
• Protein ID: WP_001261282.1
• Coordinates: 31115..31345 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM33_RS28165 (LRM33_28165)	27235..27870	+	636	WP_009309912.1	type II CAAX endopeptidase family protein	-
LRM33_RS28170 (LRM33_28170)	27978..28322	-	345	WP_009309911.1	hypothetical protein	-
LRM33_RS28175 (LRM33_28175)	28644..29174	+	531	WP_223174905.1	hypothetical protein	-
LRM33_RS28180 (LRM33_28180)	29143..29580	+	438	WP_009309910.1	hypothetical protein	-
LRM33_RS28185 (LRM33_28185)	29771..30211	-	441	WP_047057603.1	hypothetical protein	-
LRM33_RS28190 (LRM33_28190)	30230..30853	-	624	WP_223345396.1	hypothetical protein	-
LRM33_RS28195 (LRM33_28195)	30922..31158	-	237	Protein_36	hypothetical protein	-
LRM33_RS28200 (LRM33_28200)	31115..31345	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LRM33_RS28205 (LRM33_28205)	31342..31758	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRM33_RS28210 (LRM33_28210)	31832..33394	+	1563	WP_241462234.1	AAA family ATPase	-
LRM33_RS28215 (LRM33_28215)	33379..34401	+	1023	WP_000361404.1	helicase UvrD	-
LRM33_RS28220 (LRM33_28220)	34945..35853	+	909	WP_032425603.1	HNH endonuclease	-
LRM33_RS28225 (LRM33_28225)	36039..36389	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-10 / blaTEM-1B / floR / tet(A) / qnrB52 / sul1	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..186056	186056

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T226691 WP_001044770.1 NZ_CP088124:31342-31758 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T226691 NZ_CP122600:c4935751-4935440 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT226691 WP_001261282.1 NZ_CP088124:31115-31345 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT226691 NZ_CP122600:c4935439-4935149 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3