Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 21636..22279 | Replicon | plasmid pKP46-mcr10 |
| Accession | NZ_CP088124 | ||
| Organism | Klebsiella pneumoniae strain 46 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | Q84A06 |
| Locus tag | LRM33_RS28145 | Protein ID | WP_000754566.1 |
| Coordinates | 21863..22279 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | Q84A07 |
| Locus tag | LRM33_RS28140 | Protein ID | WP_001261276.1 |
| Coordinates | 21636..21866 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LRM33_RS28100 (LRM33_28100) | 17173..17286 | + | 114 | WP_014343462.1 | hypothetical protein | - |
| LRM33_RS28105 (LRM33_28105) | 17415..17672 | + | 258 | WP_009310077.1 | hypothetical protein | - |
| LRM33_RS28110 (LRM33_28110) | 17730..18509 | - | 780 | WP_023287113.1 | site-specific integrase | - |
| LRM33_RS28115 (LRM33_28115) | 18707..19723 | - | 1017 | WP_009309921.1 | hypothetical protein | - |
| LRM33_RS28120 (LRM33_28120) | 19757..20092 | - | 336 | WP_009309920.1 | hypothetical protein | - |
| LRM33_RS28125 (LRM33_28125) | 20142..20453 | - | 312 | WP_009309919.1 | hypothetical protein | - |
| LRM33_RS28130 (LRM33_28130) | 20511..20816 | - | 306 | WP_004197633.1 | type II toxin-antitoxin system toxin CcdB | - |
| LRM33_RS28135 (LRM33_28135) | 20818..21036 | - | 219 | WP_006788213.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| LRM33_RS28140 (LRM33_28140) | 21636..21866 | + | 231 | WP_001261276.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| LRM33_RS28145 (LRM33_28145) | 21863..22279 | + | 417 | WP_000754566.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| LRM33_RS28150 (LRM33_28150) | 22320..23198 | - | 879 | WP_032427228.1 | restriction endonuclease | - |
| LRM33_RS28155 (LRM33_28155) | 23860..25137 | + | 1278 | WP_009309915.1 | HlyD family secretion protein | - |
| LRM33_RS28160 (LRM33_28160) | 25127..27235 | + | 2109 | WP_032427226.1 | peptidase domain-containing ABC transporter | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | mcr-10 / blaTEM-1B / floR / tet(A) / qnrB52 / sul1 | mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ | 1..186056 | 186056 | |
| - | flank | IS/Tn | - | - | 16090..17070 | 980 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15006.52 Da Isoelectric Point: 9.2957
>T226690 WP_000754566.1 NZ_CP088124:21863-22279 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK
Download Length: 417 bp
>T226690 NZ_CP122600:c4733814-4733428 [Escherichia coli]
GTGCTTGCCCCAACTGGGCCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCCGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATATCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA
GTGCTTGCCCCAACTGGGCCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCCGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATATCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8721.74 Da Isoelectric Point: 4.3533
>AT226690 WP_001261276.1 NZ_CP088124:21636-21866 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP
Download Length: 231 bp
>AT226690 NZ_CP122600:c4734353-4733844 [Escherichia coli]
ATGGCATATCTGAAATTGATGAAGCTGCTCTATCTGTCTAACCGCAAGTCGATTTTGAAGCATGGCAGGATGATCGGCGA
AGATAGCCTTTACTCTATGAAATTTGGTCCAGTCATGTCGAATACGCTGAACTTGATTCGTGGTAAGGCTGAAGGTATTG
GTGACTACTGGTACAACCTGATAGAGACGAACGGGCATGATGTGTTGTTGCGTTCAGATCCGAGGGAAATGGATGCAGAC
GAGGTCTTTGATGAGTTGAGCCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCTGTATGGGCATATGAACCGATT
TGATCTCGCAAACATGACGCATTTAGAAAGCGTTTGTCCAGAGTGGCACAATCCTGGCAATTCTCGTAAGCCTATAGACC
TGAAAGAAATGCTGATCAGTGAGGGTAAAAGCGAGGATGAGGCTAATCGTATAATTGGCAAAATGGAAGAATCTCAGAAA
CTTAAGGAATTTTCTTTGCAATTATCATGA
ATGGCATATCTGAAATTGATGAAGCTGCTCTATCTGTCTAACCGCAAGTCGATTTTGAAGCATGGCAGGATGATCGGCGA
AGATAGCCTTTACTCTATGAAATTTGGTCCAGTCATGTCGAATACGCTGAACTTGATTCGTGGTAAGGCTGAAGGTATTG
GTGACTACTGGTACAACCTGATAGAGACGAACGGGCATGATGTGTTGTTGCGTTCAGATCCGAGGGAAATGGATGCAGAC
GAGGTCTTTGATGAGTTGAGCCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCTGTATGGGCATATGAACCGATT
TGATCTCGCAAACATGACGCATTTAGAAAGCGTTTGTCCAGAGTGGCACAATCCTGGCAATTCTCGTAAGCCTATAGACC
TGAAAGAAATGCTGATCAGTGAGGGTAAAAGCGAGGATGAGGCTAATCGTATAATTGGCAAAATGGAAGAATCTCAGAAA
CTTAAGGAATTTTCTTTGCAATTATCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A387K1G3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A387K023 |