Detailed information of TA system TA022669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3069593..3070298
• Replicon: chromosome
• Accession: NC_010468
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: ECOLC_RS15065
• Protein ID: WP_000539521.1
• Coordinates: 3069593..3069979 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ECOLC_RS15070
• Protein ID: WP_001280945.1
• Coordinates: 3069969..3070298 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECOLC_RS15045	3065597..3066223	+	627	WP_001295292.1	glutathione S-transferase GstB	-
ECOLC_RS15050	3066220..3067335	-	1116	WP_000555031.1	aldose sugar dehydrogenase YliI	-
ECOLC_RS15055	3067446..3067829	-	384	WP_000497137.1	biofilm formation regulator BssR	-
ECOLC_RS15060	3068042..3069367	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
ECOLC_RS15065	3069593..3069979	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECOLC_RS15070	3069969..3070298	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
ECOLC_RS15075	3070368..3071696	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
ECOLC_RS15080	3071704..3074052	-	2349	WP_012304894.1	EAL domain-containing protein	-
ECOLC_RS15085	3074230..3075141	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T22669 WP_000539521.1 NC_010468:3069593-3069979 [Escherichia coli ATCC 8739]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T22669 NC_010468:3069593-3069979 [Escherichia coli ATCC 8739]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT22669 WP_001280945.1 NC_010468:3069969-3070298 [Escherichia coli ATCC 8739]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT22669 NC_010468:3069969-3070298 [Escherichia coli ATCC 8739]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6