Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 20511..21036 Replicon plasmid pKP46-mcr10
Accession NZ_CP088124
Organism Klebsiella pneumoniae strain 46

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag LRM33_RS28130 Protein ID WP_004197633.1
Coordinates 20511..20816 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag LRM33_RS28135 Protein ID WP_006788213.1
Coordinates 20818..21036 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM33_RS28095 (LRM33_28095) 16090..17070 - 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -
LRM33_RS28100 (LRM33_28100) 17173..17286 + 114 WP_014343462.1 hypothetical protein -
LRM33_RS28105 (LRM33_28105) 17415..17672 + 258 WP_009310077.1 hypothetical protein -
LRM33_RS28110 (LRM33_28110) 17730..18509 - 780 WP_023287113.1 site-specific integrase -
LRM33_RS28115 (LRM33_28115) 18707..19723 - 1017 WP_009309921.1 hypothetical protein -
LRM33_RS28120 (LRM33_28120) 19757..20092 - 336 WP_009309920.1 hypothetical protein -
LRM33_RS28125 (LRM33_28125) 20142..20453 - 312 WP_009309919.1 hypothetical protein -
LRM33_RS28130 (LRM33_28130) 20511..20816 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
LRM33_RS28135 (LRM33_28135) 20818..21036 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LRM33_RS28140 (LRM33_28140) 21636..21866 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin -
LRM33_RS28145 (LRM33_28145) 21863..22279 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin -
LRM33_RS28150 (LRM33_28150) 22320..23198 - 879 WP_032427228.1 restriction endonuclease -
LRM33_RS28155 (LRM33_28155) 23860..25137 + 1278 WP_009309915.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-10 / blaTEM-1B / floR / tet(A) / qnrB52 / sul1 mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..186056 186056
- flank IS/Tn - - 16090..17070 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T226689 WP_004197633.1 NZ_CP088124:c20816-20511 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T226689 NZ_CP122600:c4601528-4601355 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT226689 WP_006788213.1 NZ_CP088124:c21036-20818 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT226689 NZ_CP122600:c4601852-4601556 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References