Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 72144..72787 Replicon plasmid pKP46-5
Accession NZ_CP088123
Organism Klebsiella pneumoniae strain 46

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag LRM33_RS27960 Protein ID WP_000754566.1
Coordinates 72371..72787 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LRM33_RS27955 Protein ID WP_001261276.1
Coordinates 72144..72374 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM33_RS27920 (LRM33_27920) 67263..67532 + 270 WP_000339857.1 hypothetical protein -
LRM33_RS27925 (LRM33_27925) 67709..68575 - 867 WP_004118283.1 replication initiation protein -
LRM33_RS27930 (LRM33_27930) 69105..69209 + 105 WP_032409716.1 hypothetical protein -
LRM33_RS27935 (LRM33_27935) 69338..69595 + 258 WP_000764642.1 hypothetical protein -
LRM33_RS27940 (LRM33_27940) 69653..70429 - 777 WP_000015958.1 site-specific integrase -
LRM33_RS27945 (LRM33_27945) 70426..71169 - 744 WP_000129823.1 hypothetical protein -
LRM33_RS27950 (LRM33_27950) 71220..71570 - 351 WP_000493378.1 hypothetical protein -
LRM33_RS27955 (LRM33_27955) 72144..72374 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LRM33_RS27960 (LRM33_27960) 72371..72787 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
LRM33_RS27965 (LRM33_27965) 72991..73847 + 857 Protein_80 IS3-like element ISEc15 family transposase -
LRM33_RS27970 (LRM33_27970) 74129..75205 + 1077 WP_000227969.1 IS110 family transposase -
LRM33_RS27975 (LRM33_27975) 75225..75326 - 102 Protein_82 Tn3 family transposase -
LRM33_RS27980 (LRM33_27980) 75846..75929 + 84 Protein_83 SOS response-associated peptidase -
LRM33_RS27985 (LRM33_27985) 75987..76955 - 969 WP_000654802.1 IS5-like element IS903B family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / mph(A) / tet(A) - 1..82962 82962


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T226688 WP_000754566.1 NZ_CP088123:72371-72787 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T226688 NZ_CP122600:4585716-4586093 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCAGCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT226688 WP_001261276.1 NZ_CP088123:72144-72374 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT226688 NZ_CP122600:4585258-4585626 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCGTCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAAGGGAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTACTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References