Detailed information of TA system TA226687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 195012..195534
• Replicon: plasmid pKP46-3
• Accession: NZ_CP088121
• Organism: Klebsiella pneumoniae strain 46

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: LRM33_RS26790
• Protein ID: WP_004181778.1
• Coordinates: 195250..195534 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: LRM33_RS26785
• Protein ID: WP_004181777.1
• Coordinates: 195012..195260 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM33_RS26765 (LRM33_26765)	190221..191042	-	822	WP_004181772.1	hypothetical protein	-
LRM33_RS26770 (LRM33_26770)	191104..191457	-	354	WP_004181774.1	hypothetical protein	-
LRM33_RS26775 (LRM33_26775)	191602..192588	-	987	WP_094965947.1	hypothetical protein	-
LRM33_RS26780 (LRM33_26780)	192922..194721	-	1800	WP_004181776.1	ATP-dependent helicase	-
LRM33_RS26785 (LRM33_26785)	195012..195260	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LRM33_RS26790 (LRM33_26790)	195250..195534	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRM33_RS26795 (LRM33_26795)	195551..195652	-	102	Protein_218	IS200/IS605 family transposase	-
LRM33_RS26800 (LRM33_26800)	195688..196914	+	1227	WP_040209639.1	RNA-guided endonuclease TnpB family protein	-
LRM33_RS26805 (LRM33_26805)	197185..197409	-	225	Protein_220	transposase	-
LRM33_RS26810 (LRM33_26810)	197488..197916	-	429	WP_077257669.1	IS200/IS605 family transposase	-
LRM33_RS26815 (LRM33_26815)	197952..199139	+	1188	WP_040209642.1	RNA-guided endonuclease TnpB family protein	-
LRM33_RS26820 (LRM33_26820)	199184..199555	-	372	WP_040209644.1	hypothetical protein	-
LRM33_RS26825 (LRM33_26825)	199552..199896	-	345	WP_223811496.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4	-	1..272060	272060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T226687 WP_004181778.1 NZ_CP088121:195250-195534 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T226687 NZ_CP122600:c4369999-4369712 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT226687 WP_004181777.1 NZ_CP088121:195012-195260 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT226687 NZ_CP122600:c4369725-4369423 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A2J4R0U8