Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4694051..4694567 Replicon chromosome
Accession NZ_CP088120
Organism Klebsiella pneumoniae strain 46

Toxin (Protein)


Gene name relE Uniprot ID J2XDK6
Locus tag LRM33_RS23030 Protein ID WP_002886902.1
Coordinates 4694051..4694335 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID R4Y888
Locus tag LRM33_RS23035 Protein ID WP_002886901.1
Coordinates 4694325..4694567 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM33_RS23005 (LRM33_23005) 4689535..4689843 - 309 WP_002886907.1 PTS sugar transporter subunit IIB -
LRM33_RS23010 (LRM33_23010) 4689928..4690101 + 174 WP_002886906.1 hypothetical protein -
LRM33_RS23015 (LRM33_23015) 4690104..4690847 + 744 WP_002886905.1 MurR/RpiR family transcriptional regulator -
LRM33_RS23020 (LRM33_23020) 4691204..4693342 + 2139 WP_002886904.1 anaerobic ribonucleoside-triphosphate reductase -
LRM33_RS23025 (LRM33_23025) 4693583..4694047 + 465 WP_002886903.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
LRM33_RS23030 (LRM33_23030) 4694051..4694335 - 285 WP_002886902.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LRM33_RS23035 (LRM33_23035) 4694325..4694567 - 243 WP_002886901.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
LRM33_RS23040 (LRM33_23040) 4694645..4696555 - 1911 WP_004152270.1 PRD domain-containing protein -
LRM33_RS23045 (LRM33_23045) 4696578..4697732 - 1155 WP_002886900.1 lactonase family protein -
LRM33_RS23050 (LRM33_23050) 4697798..4698538 - 741 WP_002886899.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11125.95 Da        Isoelectric Point: 10.3787

>T226683 WP_002886902.1 NZ_CP088120:c4694335-4694051 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESARLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Download         Length: 285 bp

>T226683 NZ_CP122600:3314671-3315057 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCACTAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9045.55 Da        Isoelectric Point: 6.3340

>AT226683 WP_002886901.1 NZ_CP088120:c4694567-4694325 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Download         Length: 243 bp

>AT226683 NZ_CP122600:3315047-3315376 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATATCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GMH2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLP0

References