Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3976730..3977349 Replicon chromosome
Accession NZ_CP088120
Organism Klebsiella pneumoniae strain 46

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LRM33_RS19585 Protein ID WP_002892050.1
Coordinates 3977131..3977349 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LRM33_RS19580 Protein ID WP_002892066.1
Coordinates 3976730..3977104 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM33_RS19570 (LRM33_19570) 3971882..3973075 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LRM33_RS19575 (LRM33_19575) 3973098..3976244 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LRM33_RS19580 (LRM33_19580) 3976730..3977104 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LRM33_RS19585 (LRM33_19585) 3977131..3977349 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LRM33_RS19590 (LRM33_19590) 3977508..3978074 + 567 WP_002892030.1 maltose O-acetyltransferase -
LRM33_RS19595 (LRM33_19595) 3978046..3978186 - 141 WP_004147370.1 hypothetical protein -
LRM33_RS19600 (LRM33_19600) 3978207..3978677 + 471 WP_002892026.1 YlaC family protein -
LRM33_RS19605 (LRM33_19605) 3978652..3980103 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LRM33_RS19610 (LRM33_19610) 3980204..3980902 + 699 WP_002892021.1 GNAT family protein -
LRM33_RS19615 (LRM33_19615) 3980899..3981039 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LRM33_RS19620 (LRM33_19620) 3981039..3981302 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T226681 WP_002892050.1 NZ_CP088120:3977131-3977349 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T226681 NZ_CP122600:2864626-2864733 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCGTGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT226681 WP_002892066.1 NZ_CP088120:3976730-3977104 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT226681 NZ_CP122600:c2864576-2864513 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References