Detailed information of TA system TA022668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2441417..2442055
• Replicon: chromosome
• Accession: NC_010468
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: ECOLC_RS11900
• Protein ID: WP_000813794.1
• Coordinates: 2441879..2442055 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ECOLC_RS11895
• Protein ID: WP_001270286.1
• Coordinates: 2441417..2441833 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECOLC_RS11875	2436569..2437510	-	942	WP_001251313.1	ABC transporter permease	-
ECOLC_RS11880	2437511..2438524	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
ECOLC_RS11885	2438542..2439687	-	1146	WP_000047426.1	ABC transporter substrate-binding protein	-
ECOLC_RS11890	2439932..2441338	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
ECOLC_RS11895	2441417..2441833	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ECOLC_RS11900	2441879..2442055	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ECOLC_RS11905	2442277..2442507	+	231	WP_000494244.1	YncJ family protein	-
ECOLC_RS11910	2442599..2444560	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ECOLC_RS11915	2444633..2445169	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ECOLC_RS11920	2445222..2446436	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T22668 WP_000813794.1 NC_010468:c2442055-2441879 [Escherichia coli ATCC 8739]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T22668 NC_010468:c2442055-2441879 [Escherichia coli ATCC 8739]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT22668 WP_001270286.1 NC_010468:c2441833-2441417 [Escherichia coli ATCC 8739]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT22668 NC_010468:c2441833-2441417 [Escherichia coli ATCC 8739]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB