Detailed information of TA system TA022666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2276932..2277458
• Replicon: chromosome
• Accession: NC_010468
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: ECOLC_RS11105
• Protein ID: WP_000323025.1
• Coordinates: 2277171..2277458 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: ECOLC_RS11100
• Protein ID: WP_000534858.1
• Coordinates: 2276932..2277171 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECOLC_RS11075	2272269..2272625	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
ECOLC_RS26235	2273236..2273523	+	288	Protein_2110	hypothetical protein	-
ECOLC_RS11090	2274292..2275605	+	1314	WP_000589006.1	ISNCY family transposase	-
ECOLC_RS11095	2276067..2276399	-	333	WP_001301033.1	protein FlxA	-
ECOLC_RS24235	2276602..2276907	-	306	WP_001326990.1	hypothetical protein	-
ECOLC_RS11100	2276932..2277171	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
ECOLC_RS11105	2277171..2277458	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
ECOLC_RS11110	2277530..2277685	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
ECOLC_RS11115	2277902..2278153	+	252	WP_000980994.1	hypothetical protein	-
ECOLC_RS11120	2278220..2278498	+	279	WP_012304870.1	hypothetical protein	-
ECOLC_RS11125	2278500..2279549	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
ECOLC_RS11130	2279563..2280315	+	753	WP_001047134.1	antitermination protein	-
ECOLC_RS25820	2280593..2280682	-	90	WP_120795389.1	hypothetical protein	-
ECOLC_RS11135	2280737..2280949	-	213	WP_000087756.1	cold shock-like protein CspF	-
ECOLC_RS11140	2281250..2281465	+	216	WP_000066484.1	cold shock-like protein CspB	-
ECOLC_RS11145	2282219..2282434	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2252029..2319764	67735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T22666 WP_000323025.1 NC_010468:2277171-2277458 [Escherichia coli ATCC 8739]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T22666 NC_010468:2277171-2277458 [Escherichia coli ATCC 8739]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT22666 WP_000534858.1 NC_010468:2276932-2277171 [Escherichia coli ATCC 8739]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT22666 NC_010468:2276932-2277171 [Escherichia coli ATCC 8739]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8