Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 2458654..2459297 | Replicon | chromosome |
| Accession | NZ_CP088119 | ||
| Organism | Enterobacter kobei strain 6 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | A0A155IU92 |
| Locus tag | LRM31_RS11810 | Protein ID | WP_000754567.1 |
| Coordinates | 2458881..2459297 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | A0A155IUD1 |
| Locus tag | LRM31_RS11805 | Protein ID | WP_023293777.1 |
| Coordinates | 2458654..2458884 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LRM31_RS11775 (LRM31_11775) | 2453710..2454141 | + | 432 | WP_006796638.1 | translesion error-prone DNA polymerase V autoproteolytic subunit | - |
| LRM31_RS11780 (LRM31_11780) | 2454141..2455412 | + | 1272 | WP_032627083.1 | Y-family DNA polymerase | - |
| LRM31_RS11785 (LRM31_11785) | 2455494..2456468 | - | 975 | WP_000064119.1 | ParB family protein | - |
| LRM31_RS11790 (LRM31_11790) | 2456468..2457673 | - | 1206 | WP_006797591.1 | AAA family ATPase | - |
| LRM31_RS11795 (LRM31_11795) | 2457803..2457874 | + | 72 | Protein_2308 | hypothetical protein | - |
| LRM31_RS11800 (LRM31_11800) | 2457962..2458516 | - | 555 | WP_161646454.1 | hypothetical protein | - |
| LRM31_RS11805 (LRM31_11805) | 2458654..2458884 | + | 231 | WP_023293777.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| LRM31_RS11810 (LRM31_11810) | 2458881..2459297 | + | 417 | WP_000754567.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| LRM31_RS11815 (LRM31_11815) | 2459648..2460616 | - | 969 | WP_000654804.1 | IS5-like element IS903B family transposase | - |
| LRM31_RS11820 (LRM31_11820) | 2460742..2461443 | - | 702 | WP_008502228.1 | DsbA family oxidoreductase | - |
| LRM31_RS11825 (LRM31_11825) | 2461509..2462615 | - | 1107 | WP_006687059.1 | alkene reductase | - |
| LRM31_RS11830 (LRM31_11830) | 2462828..2463157 | + | 330 | WP_008502229.1 | thioredoxin family protein | - |
| LRM31_RS11835 (LRM31_11835) | 2463187..2463525 | - | 339 | WP_000888080.1 | carboxymuconolactone decarboxylase family protein | - |
| LRM31_RS11840 (LRM31_11840) | 2463530..2464111 | - | 582 | WP_008502230.1 | TetR/AcrR family transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 2450866..2474973 | 24107 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15108.61 Da Isoelectric Point: 8.5403
>T226658 WP_000754567.1 NZ_CP088119:2458881-2459297 [Enterobacter kobei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK
Download Length: 417 bp
>T226658 NZ_CP122588:c4506591-4506241 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8706.73 Da Isoelectric Point: 4.4299
>AT226658 WP_023293777.1 NZ_CP088119:2458654-2458884 [Enterobacter kobei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP
Download Length: 231 bp
>AT226658 NZ_CP122588:c4506836-4506585 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTCAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTCAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A155IU92 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A155IUD1 |