Detailed information of TA system TA226657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1775730..1776527
• Replicon: chromosome
• Accession: NZ_CP088119
• Organism: Enterobacter kobei strain 6

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: LRM31_RS08405
• Protein ID: WP_176450254.1
• Coordinates: 1776003..1776527 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A2W0G3H3
• Locus tag: LRM31_RS08400
• Protein ID: WP_014883303.1
• Coordinates: 1775730..1775999 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM31_RS08370 (LRM31_08370)	1771351..1771851	+	501	WP_014883299.1	DedA family protein	-
LRM31_RS08375 (LRM31_08375)	1771955..1773196	+	1242	WP_023337066.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
LRM31_RS08380 (LRM31_08380)	1773230..1773457	-	228	WP_014883300.1	YccJ family protein	-
LRM31_RS08385 (LRM31_08385)	1773476..1774072	-	597	WP_014883301.1	NAD(P)H:quinone oxidoreductase	-
LRM31_RS08390 (LRM31_08390)	1774464..1774634	+	171	WP_023332656.1	general stress protein	-
LRM31_RS08395 (LRM31_08395)	1774751..1775668	+	918	WP_047028038.1	DMT family transporter	-
LRM31_RS08400 (LRM31_08400)	1775730..1775999	+	270	WP_014883303.1	DUF1778 domain-containing protein	Antitoxin
LRM31_RS08405 (LRM31_08405)	1776003..1776527	+	525	WP_176450254.1	GNAT family N-acetyltransferase	Toxin
LRM31_RS08410 (LRM31_08410)	1776601..1777923	-	1323	WP_014883305.1	pyrimidine utilization transport protein G	-
LRM31_RS08415 (LRM31_08415)	1777945..1778439	-	495	WP_023332659.1	pyrimidine utilization flavin reductase protein F	-
LRM31_RS08420 (LRM31_08420)	1778449..1779039	-	591	WP_014883307.1	malonic semialdehyde reductase	-
LRM31_RS08425 (LRM31_08425)	1779049..1779849	-	801	WP_047028039.1	pyrimidine utilization protein D	-
LRM31_RS08430 (LRM31_08430)	1779857..1780243	-	387	WP_014883309.1	pyrimidine utilization protein C	-
LRM31_RS08435 (LRM31_08435)	1780255..1780944	-	690	WP_014883310.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19689.59 Da
• Isoelectric Point: 8.9464

>T226657 WP_176450254.1 NZ_CP088119:1776003-1776527 [Enterobacter kobei]
IVANLTIEMFSEGSDYDFRGFDCGEPSLNAFLTEHLVRQHKGRILRGYLLKDRDHPRVLGYYTLSGSCFEKAMLPSKTQQ
RRIPYVNVPSVTLGRLAVHKDLQGKEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNEQAKRFYLKQGFIPLTGENSHSL
FFPTKSIEQLFEQA

Nucleotide

• Length: 525 bp

>T226657 NZ_CP122588:c4398895-4398608 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCT
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10278.72 Da
• Isoelectric Point: 5.8836

>AT226657 WP_014883303.1 NZ_CP088119:1775730-1775999 [Enterobacter kobei]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNESSWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT226657 NZ_CP122588:c4398621-4398319 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGGCACCCCCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2W0G3H3