Detailed information of TA system TA226653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 581200..581939
• Replicon: chromosome
• Accession: NZ_CP088119
• Organism: Enterobacter kobei strain 6

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A9E6WPH7
• Locus tag: LRM31_RS02800
• Protein ID: WP_013087269.1
• Coordinates: 581454..581939 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A9E6WPN8
• Locus tag: LRM31_RS02795
• Protein ID: WP_007869691.1
• Coordinates: 581200..581466 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRM31_RS02775 (LRM31_02775)	577198..578871	+	1674	WP_241448973.1	methyl-accepting chemotaxis protein	-
LRM31_RS02780 (LRM31_02780)	578903..579310	-	408	Protein_538	EamA/RhaT family transporter	-
LRM31_RS02785 (LRM31_02785)	579366..580063	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
LRM31_RS02790 (LRM31_02790)	580080..580547	-	468	Protein_540	plasmid replication initiator RepA	-
LRM31_RS02795 (LRM31_02795)	581200..581466	+	267	WP_007869691.1	DUF1778 domain-containing protein	Antitoxin
LRM31_RS02800 (LRM31_02800)	581454..581939	+	486	WP_013087269.1	GNAT family N-acetyltransferase	Toxin
LRM31_RS02805 (LRM31_02805)	582146..583491	+	1346	Protein_543	ISNCY family transposase	-
LRM31_RS02810 (LRM31_02810)	583675..583791	-	117	Protein_544	transposase domain-containing protein	-
LRM31_RS02815 (LRM31_02815)	583736..583999	-	264	WP_004118209.1	transposase	-
LRM31_RS02820 (LRM31_02820)	584014..584277	+	264	WP_001295708.1	hypothetical protein	-
LRM31_RS02825 (LRM31_02825)	584519..584800	+	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
LRM31_RS02830 (LRM31_02830)	584835..585404	+	570	WP_004118809.1	small heat shock protein sHSP20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	573101..635315	62214
	-	inside	IScluster/Tn	-	-	572645..583491	10846

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17736.45 Da
• Isoelectric Point: 9.5181

>T226653 WP_013087269.1 NZ_CP088119:581454-581939 [Enterobacter kobei]
VGRVTAPEPLSSSHQLAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKRDTKQVVGFYSLATGSVNHVEAMGSLRRNM
PDPIPVIILARLAVDVSYRGQGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYLHHGFKASQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T226653 NZ_CP122588:3754261-3754479 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10080.46 Da
• Isoelectric Point: 4.3837

>AT226653 WP_007869691.1 NZ_CP088119:581200-581466 [Enterobacter kobei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVSDEAAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT226653 NZ_CP122588:3753861-3754235 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG