Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 7020..7555 Replicon plasmid pRRI128_3
Accession NZ_CP088116
Organism Sinorhizobium meliloti strain RRI128

Toxin (Protein)


Gene name relE Uniprot ID A0A6G1WU13
Locus tag SmelRRI128_RS31640 Protein ID WP_024325581.1
Coordinates 7020..7295 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2N8JT21
Locus tag SmelRRI128_RS31645 Protein ID WP_013845222.1
Coordinates 7295..7555 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SmelRRI128_RS31615 (SmelRRI128_31615) 2481..3695 + 1215 WP_027991198.1 plasmid replication protein RepC -
SmelRRI128_RS31620 (SmelRRI128_31620) 4664..5128 - 465 WP_231198667.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
SmelRRI128_RS31625 (SmelRRI128_31625) 5129..5398 - 270 WP_027991199.1 hypothetical protein -
SmelRRI128_RS31630 (SmelRRI128_31630) 5546..5767 + 222 WP_050579999.1 hypothetical protein -
SmelRRI128_RS31635 (SmelRRI128_31635) 5816..7039 - 1224 WP_231198686.1 site-specific integrase -
SmelRRI128_RS31640 (SmelRRI128_31640) 7020..7295 - 276 WP_024325581.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
SmelRRI128_RS31645 (SmelRRI128_31645) 7295..7555 - 261 WP_013845222.1 ribbon-helix-helix domain-containing protein Antitoxin
SmelRRI128_RS31650 (SmelRRI128_31650) 8160..9362 + 1203 WP_027991238.1 nucleotidyltransferase -
SmelRRI128_RS31655 (SmelRRI128_31655) 9359..11074 + 1716 WP_050580008.1 ThiF family adenylyltransferase -
SmelRRI128_RS31660 (SmelRRI128_31660) 11074..11532 + 459 WP_027991237.1 Mov34/MPN/PAD-1 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - htpB 1..306339 306339


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10822.40 Da        Isoelectric Point: 9.9707

>T226650 WP_024325581.1 NZ_CP088116:c7295-7020 [Sinorhizobium meliloti]
MKLEWTSKAVADLGRLYDFLAPVNRQAAARTVQSLTSAPARLLEQPRIGERLEEFDPREVRRILVGHYEIRYEIRQSTIY
VLRLWHTREDR

Download         Length: 276 bp

>T226650 NZ_CP122588:c2570256-2570080 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9652.01 Da        Isoelectric Point: 4.8182

>AT226650 WP_013845222.1 NZ_CP088116:c7555-7295 [Sinorhizobium meliloti]
METKVLTAHVPLPLAEKVDQIAARLERSRGWIVKQALTAWVDQEEERRRLTLEALADVDAGRVIDHQAVQAWADSLDSDK
PLSLPL

Download         Length: 261 bp

>AT226650 NZ_CP122588:c2570034-2569618 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G1WU13

Antitoxin

Source ID Structure
AlphaFold DB A0A2N8JT21

References