Detailed information of TA system TA226647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 680905..681590
• Replicon: plasmid pRRI128_2
• Accession: NZ_CP088115
• Organism: Sinorhizobium meliloti strain RRI128

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SmelRRI128_RS28645
• Protein ID: WP_017263896.1
• Coordinates: 680905..681324 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: SmelRRI128_RS28650
• Protein ID: WP_013845417.1
• Coordinates: 681321..681590 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SmelRRI128_RS28615 (SmelRRI128_28615)	676357..677268	+	912	WP_027991032.1	LysR family transcriptional regulator	-
SmelRRI128_RS28620 (SmelRRI128_28620)	677561..677968	-	408	Protein_640	helix-turn-helix domain-containing protein	-
SmelRRI128_RS28625 (SmelRRI128_28625)	678094..679094	-	1001	Protein_641	IS30 family transposase	-
SmelRRI128_RS28630 (SmelRRI128_28630)	679354..679734	-	381	WP_003526626.1	transcriptional regulator	-
SmelRRI128_RS28635 (SmelRRI128_28635)	679752..680063	-	312	WP_014528619.1	type II toxin-antitoxin system HigB family toxin	-
SmelRRI128_RS28640 (SmelRRI128_28640)	680338..680751	+	414	WP_014528620.1	hypothetical protein	-
SmelRRI128_RS28645 (SmelRRI128_28645)	680905..681324	-	420	WP_017263896.1	type II toxin-antitoxin system VapC family toxin	Toxin
SmelRRI128_RS28650 (SmelRRI128_28650)	681321..681590	-	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SmelRRI128_RS28655 (SmelRRI128_28655)	681728..682102	+	375	Protein_647	LysR substrate-binding domain-containing protein	-
SmelRRI128_RS28660 (SmelRRI128_28660)	682190..683065	-	876	WP_013845418.1	LysR family transcriptional regulator	-
SmelRRI128_RS28665 (SmelRRI128_28665)	684045..684968	-	924	WP_014528622.1	LysR family transcriptional regulator	-
SmelRRI128_RS28670 (SmelRRI128_28670)	685085..685954	+	870	WP_014528623.1	aldo/keto reductase family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1289339	1289339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.00 Da
• Isoelectric Point: 4.8722

>T226647 WP_017263896.1 NZ_CP088115:c681324-680905 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG

Nucleotide

• Length: 420 bp

>T226647 NZ_CP122588:2116629-2116732 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT226647 WP_013845417.1 NZ_CP088115:c681590-681321 [Sinorhizobium meliloti]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT226647 NZ_CP122588:c2116770-2116499 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5