Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 299982..300589 Replicon plasmid pRRI128_2
Accession NZ_CP088115
Organism Sinorhizobium meliloti strain RRI128

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag SmelRRI128_RS26775 Protein ID WP_017264738.1
Coordinates 300335..300589 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag SmelRRI128_RS26770 Protein ID WP_027990729.1
Coordinates 299982..300338 (-) Length 119 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SmelRRI128_RS26740 (SmelRRI128_26740) 295290..295796 + 507 WP_050579965.1 flavin reductase family protein -
SmelRRI128_RS26745 (SmelRRI128_26745) 296073..297257 - 1185 WP_027990731.1 Fic family protein -
SmelRRI128_RS26750 (SmelRRI128_26750) 297797..298282 - 486 WP_033053021.1 GNAT family N-acetyltransferase -
SmelRRI128_RS26755 (SmelRRI128_26755) 298424..299056 - 633 WP_027990730.1 LysE family translocator -
SmelRRI128_RS26760 (SmelRRI128_26760) 299084..299278 - 195 Protein_266 hypothetical protein -
SmelRRI128_RS26765 (SmelRRI128_26765) 299363..299680 + 318 WP_127641963.1 hypothetical protein -
SmelRRI128_RS26770 (SmelRRI128_26770) 299982..300338 - 357 WP_027990729.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SmelRRI128_RS26775 (SmelRRI128_26775) 300335..300589 - 255 WP_017264738.1 type II toxin-antitoxin system HicA family toxin Toxin
SmelRRI128_RS26780 (SmelRRI128_26780) 300921..301808 - 888 WP_027990728.1 LysR family transcriptional regulator -
SmelRRI128_RS26785 (SmelRRI128_26785) 301897..302937 + 1041 WP_010968044.1 NAD(P)H-binding protein -
SmelRRI128_RS26790 (SmelRRI128_26790) 303061..303219 + 159 WP_017267400.1 hypothetical protein -
SmelRRI128_RS26795 (SmelRRI128_26795) 303254..303460 + 207 WP_153495115.1 hypothetical protein -
SmelRRI128_RS26800 (SmelRRI128_26800) 303512..304384 - 873 WP_027990727.1 LysR family transcriptional regulator -
SmelRRI128_RS26805 (SmelRRI128_26805) 304475..305179 + 705 WP_027990726.1 haloacid dehalogenase type II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..1289339 1289339


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9294.62 Da        Isoelectric Point: 9.8124

>T226646 WP_017264738.1 NZ_CP088115:c300589-300335 [Sinorhizobium meliloti]
MNSKQRKTLAAVFKDPASGTIEWAAIENLLVAAGADVIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Download         Length: 255 bp

>T226646 NZ_CP122588:c1241073-1240753 [Escherichia coli]
ATGCACATTTCAACTGTACCGGCCACGGTGCCGGTTTCGTCACGTCTGTCTCCCGTGCAGGTCTGGCAGCAACTGCTAAC
ATATCTGCTGGAGCACCATTACGGACTGACGCTTAACGACACGCCATTCCATGACAACACGGCCATTGAGGAGCATATCG
ACGCAGGAATAAAGCTTGTCGATGCAGTGAATTTTCTAGTGGAACGTTATGAACTGGTACGCATCGACCGGAAGGGATTT
TCATGGCAGGATCAGACGCCATTCCTCAGCGCTACCGATATTCTCAGAGCAAGGCGAGCTACGGGGATAATTAGTACTTA
A

Antitoxin


Download         Length: 119 a.a.        Molecular weight: 13103.80 Da        Isoelectric Point: 6.3320

>AT226646 WP_027990729.1 NZ_CP088115:c300338-299982 [Sinorhizobium meliloti]
MNTMTYNGYHARIEFDAEDEVFFGKIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEKAAGHFAAARPSA

Download         Length: 357 bp

>AT226646 NZ_CP122588:c1241433-1241110 [Escherichia coli]
ATGTCGTTATCTGATTCTCCCCAATGGGGACTGCAAAGTGATATTACCCCCCGCTTTGGCGCAAGGCTGGTACAGGAGGG
CAAGCGTCTGCATTACCTAGCTGACAGGGCTGGGTTGACGGGGGGCTTTACCCCGGAGCAACTGCAGAAATTGGATCAGA
CTTTCCCGCTGTTTATAGATCAACTGGAATCAATGCTGCGTTCCGGTGAGCTAAATCCAAAGCAGCAACATCAGGTGAAC
ATTCATCTTACGGGTTTAACCTGCATAGCTGATACCCGTGGTAGCTGCGGTTATGTTTACATTTCTATTTATCTAACCCC
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References