Detailed information of TA system TA226636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 455520..456160
• Replicon: chromosome
• Accession: NZ_CP088113
• Organism: Sinorhizobium meliloti strain RRI128

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H0G6N1
• Locus tag: SmelRRI128_RS02205
• Protein ID: WP_003533691.1
• Coordinates: 455520..455906 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0G6N0
• Locus tag: SmelRRI128_RS02210
• Protein ID: WP_003533689.1
• Coordinates: 455906..456160 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SmelRRI128_RS02180 (SmelRRI128_02180)	450991..451470	+	480	WP_003537307.1	GNAT family N-acetyltransferase	-
SmelRRI128_RS02185 (SmelRRI128_02185)	451513..451962	-	450	WP_003537305.1	nucleoside deaminase	-
SmelRRI128_RS02190 (SmelRRI128_02190)	452037..453758	+	1722	WP_027989728.1	pseudouridine synthase	-
SmelRRI128_RS02195 (SmelRRI128_02195)	453765..454325	+	561	WP_027989727.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
SmelRRI128_RS02200 (SmelRRI128_02200)	454413..455297	+	885	WP_033052877.1	patatin-like phospholipase family protein	-
SmelRRI128_RS02205 (SmelRRI128_02205)	455520..455906	-	387	WP_003533691.1	type II toxin-antitoxin system VapC family toxin	Toxin
SmelRRI128_RS02210 (SmelRRI128_02210)	455906..456160	-	255	WP_003533689.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SmelRRI128_RS02215 (SmelRRI128_02215)	456298..458130	-	1833	WP_027989725.1	monovalent cation:proton antiporter-2 (CPA2) family protein	-
SmelRRI128_RS02220 (SmelRRI128_02220)	458282..459628	+	1347	WP_027989724.1	TldD/PmbA family protein	-
SmelRRI128_RS02225 (SmelRRI128_02225)	459737..460555	+	819	WP_027989723.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
SmelRRI128_RS02230 (SmelRRI128_02230)	460616..460852	+	237	WP_003533684.1	DUF4170 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13904.77 Da
• Isoelectric Point: 4.5716

>T226636 WP_003533691.1 NZ_CP088113:c455906-455520 [Sinorhizobium meliloti]
MVIDTSAIAAIAFNEQEAGSFREKIADDPVRLISAATALEAAMVIETRLGEAAGAELDLWLYKANVEIVAVTAEHMDQAR
RAWRRFGKGRHPAGLNFGDCFSYALAFLTNEPLLFKGSDFSQTDIPAA

Nucleotide

• Length: 387 bp

>T226636 NZ_CP122587:c4822451-4822167 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9472.63 Da
• Isoelectric Point: 4.8660

>AT226636 WP_003533689.1 NZ_CP088113:c456160-455906 [Sinorhizobium meliloti]
MALSIKDTETEQLARTLARRTGESITLATKRALEERLRRTDTAARRAALLEDLEAIQRRWNAMPVLDSRSSDDIIGYDED
GLPS

Nucleotide

• Length: 255 bp

>AT226636 NZ_CP122587:c4822683-4822441 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: H0G6N1

Antitoxin

• AlphaFold DB: H0G6N0