Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 89887..90452 Replicon chromosome
Accession NZ_CP088113
Organism Sinorhizobium meliloti strain RRI128

Toxin (Protein)


Gene name brnT Uniprot ID A0A843ZV60
Locus tag SmelRRI128_RS00430 Protein ID WP_013843871.1
Coordinates 90207..90452 (-) Length 82 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID H0G3P5
Locus tag SmelRRI128_RS00425 Protein ID WP_003531566.1
Coordinates 89887..90210 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SmelRRI128_RS00405 (SmelRRI128_00405) 85750..86550 + 801 WP_027989493.1 TVP38/TMEM64 family protein -
SmelRRI128_RS00410 (SmelRRI128_00410) 86573..87994 + 1422 WP_027989492.1 NAD(P)/FAD-dependent oxidoreductase -
SmelRRI128_RS00415 (SmelRRI128_00415) 88069..89589 + 1521 WP_027989491.1 HAMP domain-containing sensor histidine kinase -
SmelRRI128_RS00425 (SmelRRI128_00425) 89887..90210 - 324 WP_003531566.1 BrnA antitoxin family protein Antitoxin
SmelRRI128_RS00430 (SmelRRI128_00430) 90207..90452 - 246 WP_013843871.1 BrnT family toxin Toxin
SmelRRI128_RS00435 (SmelRRI128_00435) 90887..91600 + 714 WP_013843872.1 DNA helicase -
SmelRRI128_RS00440 (SmelRRI128_00440) 91785..92480 - 696 WP_027989490.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
SmelRRI128_RS00445 (SmelRRI128_00445) 92673..93161 + 489 WP_027989489.1 RidA family protein -
SmelRRI128_RS00450 (SmelRRI128_00450) 93370..93987 + 618 WP_027989488.1 hypothetical protein -
SmelRRI128_RS00455 (SmelRRI128_00455) 94079..95224 + 1146 WP_027989487.1 alpha-hydroxy acid oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-77)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-101)


Sequences

Toxin        


Download         Length: 82 a.a.        Molecular weight: 9004.50 Da        Isoelectric Point: 9.7323

>T226634 WP_013843871.1 NZ_CP088113:c90452-90207 [Sinorhizobium meliloti]
MKITYDETKRQINIGKHGYDIAELDMEFFASSVVVPAKEGRFMAIGVLRNGVIAVVFARLGSEGISIISMRDASRKERSL
L

Download         Length: 246 bp

>T226634 NZ_CP122587:4108019-4108237 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11933.51 Da        Isoelectric Point: 10.2830

>AT226634 WP_003531566.1 NZ_CP088113:c90210-89887 [Sinorhizobium meliloti]
MTSLSRRIRHISDKEEAAIQRKIASDPDAPEATDEQLAKAKPFAEAFPDLAESIKRARGRPAVENPRQQISIRLDPDVIE
HYKATGKGWQSRMNDDLRKAAGLKKAG

Download         Length: 324 bp

>AT226634 NZ_CP122587:4107618-4107992 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A843ZV60

Antitoxin

Source ID Structure
AlphaFold DB H0G3P5

References