Detailed information of TA system TA226626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 270500..271176
• Replicon: plasmid pTA1_1
• Accession: NZ_CP088105
• Organism: Rhizobium ruizarguesonis strain TA1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RlegTA1_RS26060
• Protein ID: WP_027688084.1
• Coordinates: 270751..271176 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4Q8ZXM1
• Locus tag: RlegTA1_RS26055
• Protein ID: WP_075224380.1
• Coordinates: 270500..270754 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RlegTA1_RS26025 (RlegTA1_26025)	265854..266747	-	894	WP_027688088.1	NmrA family NAD(P)-binding protein	-
RlegTA1_RS26030 (RlegTA1_26030)	266815..267240	-	426	WP_027688087.1	MarR family winged helix-turn-helix transcriptional regulator	-
RlegTA1_RS26035 (RlegTA1_26035)	267672..267842	+	171	WP_231286869.1	hypothetical protein	-
RlegTA1_RS26040 (RlegTA1_26040)	267916..268881	-	966	WP_027688086.1	alpha/beta hydrolase	-
RlegTA1_RS26045 (RlegTA1_26045)	268978..269664	-	687	WP_027688085.1	glutathione S-transferase	-
RlegTA1_RS26050 (RlegTA1_26050)	269769..270344	+	576	WP_018480381.1	TetR/AcrR family transcriptional regulator	-
RlegTA1_RS26055 (RlegTA1_26055)	270500..270754	+	255	WP_075224380.1	plasmid stabilization protein	Antitoxin
RlegTA1_RS26060 (RlegTA1_26060)	270751..271176	+	426	WP_027688084.1	type II toxin-antitoxin system VapC family toxin	Toxin
RlegTA1_RS26065 (RlegTA1_26065)	271622..272410	+	789	WP_027688083.1	siderophore-iron reductase FhuF	-
RlegTA1_RS26070 (RlegTA1_26070)	272528..273205	-	678	Protein_256	phosphopantetheinyl transferase	-
RlegTA1_RS26075 (RlegTA1_26075)	273593..275470	+	1878	WP_024321535.1	HAMP domain-containing methyl-accepting chemotaxis protein	-
RlegTA1_RS26080 (RlegTA1_26080)	275667..276056	+	390	WP_027688081.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..805037	805037

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15186.42 Da
• Isoelectric Point: 4.8283

>T226626 WP_027688084.1 NZ_CP088105:270751-271176 [Rhizobium ruizarguesonis]
MILIDTNVISEPWKPTPDNQVLAWIDAQAVETLFVSAITVAELRFGIAAMPSGRRKTVLDDRLEQEVLPLFSGRVLSFDM
AASQSYSELMARARAAGRAIGKADAYIAATAAANGFIVATRDSSPFEAAGLKVINPWYAPT

Nucleotide

• Length: 426 bp

>T226626 NZ_CP122583:c6121-5816 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9249.43 Da
• Isoelectric Point: 5.6158

>AT226626 WP_075224380.1 NZ_CP088105:270500-270754 [Rhizobium ruizarguesonis]
MAAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNDDVDNLQLVRDKTPAEP
MSFE

Nucleotide

• Length: 255 bp

>AT226626 NZ_CP122583:c6341-6123 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A4Q8ZXM1