Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 60974..61665 Replicon plasmid pTA1_1
Accession NZ_CP088105
Organism Rhizobium ruizarguesonis strain TA1

Toxin (Protein)


Gene name vapC Uniprot ID A0A4Q8ZX68
Locus tag RlegTA1_RS25080 Protein ID WP_027688184.1
Coordinates 60974..61402 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A4R0AD13
Locus tag RlegTA1_RS25085 Protein ID WP_017991284.1
Coordinates 61399..61665 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RlegTA1_RS25060 (RlegTA1_25060) 57869..58708 + 840 WP_027688185.1 transporter substrate-binding domain-containing protein -
RlegTA1_RS25065 (RlegTA1_25065) 58826..59488 + 663 WP_003550217.1 amino acid ABC transporter permease -
RlegTA1_RS25070 (RlegTA1_25070) 59495..60154 + 660 WP_024319797.1 amino acid ABC transporter permease -
RlegTA1_RS25075 (RlegTA1_25075) 60167..60937 + 771 WP_018480586.1 amino acid ABC transporter ATP-binding protein -
RlegTA1_RS25080 (RlegTA1_25080) 60974..61402 - 429 WP_027688184.1 type II toxin-antitoxin system VapC family toxin Toxin
RlegTA1_RS25085 (RlegTA1_25085) 61399..61665 - 267 WP_017991284.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RlegTA1_RS25090 (RlegTA1_25090) 61922..62665 + 744 WP_027688183.1 GntR family transcriptional regulator -
RlegTA1_RS25095 (RlegTA1_25095) 62669..63454 + 786 WP_024319800.1 transporter substrate-binding domain-containing protein -
RlegTA1_RS25100 (RlegTA1_25100) 63536..64207 + 672 WP_024319801.1 amino acid ABC transporter permease -
RlegTA1_RS25105 (RlegTA1_25105) 64204..64863 + 660 WP_024319802.1 amino acid ABC transporter permease -
RlegTA1_RS25110 (RlegTA1_25110) 64841..65566 + 726 WP_024319803.1 amino acid ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..805037 805037


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-66)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15824.14 Da        Isoelectric Point: 4.7948

>T226625 WP_027688184.1 NZ_CP088105:c61402-60974 [Rhizobium ruizarguesonis]
VRLLLDTNVLSEVTRPRPDAHVLQWLDDLDEDRSFISVVSIAEIRRGVALMESGRKRDALAEWLAQDLPQRFEQRVIPVD
QAVAITWGDLMGLAKRSGRGLSSMGGLIAATAIAHDLTLATRNIKDFESLGIELVDPWIERP

Download         Length: 429 bp

>T226625 NZ_CP122581:c14266-13916 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9960.34 Da        Isoelectric Point: 10.0842

>AT226625 WP_017991284.1 NZ_CP088105:c61665-61399 [Rhizobium ruizarguesonis]
MQPSRPEDKSWTVASAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLRGADLDLERQR
DEPRDLQL

Download         Length: 267 bp

>AT226625 NZ_CP122581:c13911-13609 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q8ZX68

Antitoxin

Source ID Structure
AlphaFold DB A0A4R0AD13

References