Detailed information of TA system TA226622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 123707..124380
• Replicon: plasmid pCC829_2
• Accession: NZ_CP088102
• Organism: Bradyrhizobium japonicum strain CC829

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BjapCC829_RS48670
• Protein ID: WP_231145931.1
• Coordinates: 123707..124075 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BjapCC829_RS48675
• Protein ID: WP_085398373.1
• Coordinates: 124072..124380 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BjapCC829_RS48650 (BjapCC829_48650)	119826..120461	+	636	WP_231145929.1	response regulator transcription factor	-
BjapCC829_RS48655 (BjapCC829_48655)	120543..120884	+	342	WP_231145930.1	hypothetical protein	-
BjapCC829_RS48660 (BjapCC829_48660)	120884..121933	+	1050	WP_231145965.1	patatin-like phospholipase family protein	-
BjapCC829_RS48665 (BjapCC829_48665)	121954..123408	+	1455	WP_231145966.1	GntP family permease	-
BjapCC829_RS48670 (BjapCC829_48670)	123707..124075	-	369	WP_231145931.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BjapCC829_RS48675 (BjapCC829_48675)	124072..124380	-	309	WP_085398373.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BjapCC829_RS48680 (BjapCC829_48680)	124515..124826	-	312	WP_028343038.1	DUF736 domain-containing protein	-
BjapCC829_RS48685 (BjapCC829_48685)	125214..126735	+	1522	Protein_135	IS21 family transposase	-
BjapCC829_RS48690 (BjapCC829_48690)	126713..127435	+	723	Protein_136	IS21-like element helper ATPase IstB	-
BjapCC829_RS48695 (BjapCC829_48695)	127571..128271	-	701	Protein_137	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..335549	335549
	-	flank	IS/Tn	-	-	118172..119506	1334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-69)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-58)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13638.75 Da
• Isoelectric Point: 7.8253

>T226622 WP_231145931.1 NZ_CP088102:c124075-123707 [Bradyrhizobium japonicum]
VKLVWSRFALFDRDGIFSYIEAENPRAAVHVDEQIANAARRLLDFPDSGRPGRVAGTRELVIPRTSYVIEILVPPESLPI
LEWLGIKTSKSPLIVRSCVDLEQLACRQPVAYAVTDTRRGPP

Nucleotide

• Length: 369 bp

>T226622 NZ_CP122579:47286-47768 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11424.89 Da
• Isoelectric Point: 4.8950

>AT226622 WP_085398373.1 NZ_CP088102:c124380-124072 [Bradyrhizobium japonicum]
MASNALVQTRIDADVKEKATAVLENMGLTVSDVVRILLTRTANEGMLPLELVSNSQAYDTWFREKVRQALADTRPDIDDS
EVEAHFAQRRAAALRKAPEQER

Nucleotide

• Length: 309 bp

>AT226622 NZ_CP122579:47032-47298 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA