Detailed information of TA system TA226621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 2159956..2160659
• Replicon: chromosome
• Accession: NZ_CP088100
• Organism: Bradyrhizobium japonicum strain CC829

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BjapCC829_RS09975
• Protein ID: WP_063985190.1
• Coordinates: 2159956..2160381 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8T5VAC8
• Locus tag: BjapCC829_RS09980
• Protein ID: WP_028148610.1
• Coordinates: 2160378..2160659 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BjapCC829_RS09945 (BjapCC829_09945)	2155888..2156298	+	411	WP_063985193.1	acyl-CoA thioesterase	-
BjapCC829_RS09950 (BjapCC829_09950)	2156519..2156710	+	192	WP_028134810.1	hypothetical protein	-
BjapCC829_RS09955 (BjapCC829_09955)	2156976..2157371	-	396	WP_155795382.1	nuclear transport factor 2 family protein	-
BjapCC829_RS09960 (BjapCC829_09960)	2157691..2158158	-	468	WP_231144343.1	nuclear transport factor 2 family protein	-
BjapCC829_RS09965 (BjapCC829_09965)	2158313..2159011	+	699	WP_063985192.1	HAD-IA family hydrolase	-
BjapCC829_RS09970 (BjapCC829_09970)	2159103..2159951	+	849	WP_063985191.1	DeoR/GlpR family DNA-binding transcription regulator	-
BjapCC829_RS09975 (BjapCC829_09975)	2159956..2160381	-	426	WP_063985190.1	type II toxin-antitoxin system VapC family toxin	Toxin
BjapCC829_RS09980 (BjapCC829_09980)	2160378..2160659	-	282	WP_028148610.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BjapCC829_RS09985 (BjapCC829_09985)	2160889..2162439	+	1551	WP_063985189.1	glycerol-3-phosphate dehydrogenase	-
BjapCC829_RS09990 (BjapCC829_09990)	2162436..2163512	+	1077	WP_231144344.1	ABC transporter ATP-binding protein	-
BjapCC829_RS09995 (BjapCC829_09995)	2163527..2164612	+	1086	WP_231144345.1	ABC transporter ATP-binding protein	-
BjapCC829_RS10000 (BjapCC829_10000)	2164612..2165586	+	975	WP_063985186.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (14-77)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15471.74 Da
• Isoelectric Point: 5.1538

>T226621 WP_063985190.1 NZ_CP088100:c2160381-2159956 [Bradyrhizobium japonicum]
VNLLLDTNVLSEVQRPAPSPKVLAWLDTVDEDRTFISVASIAELRRGIALLDDGRRRAALVAWLAHDLPARFAQRILPID
HAVAERWGDLMAQSRRNGVALSVMDGFFAATALANDLTLVTRNVKDFAAFGVPLLDPWSDA

Nucleotide

• Length: 426 bp

>T226621 NZ_CP122578:c5206983-5206600 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10250.47 Da
• Isoelectric Point: 5.1409

>AT226621 WP_028148610.1 NZ_CP088100:c2160659-2160378 [Bradyrhizobium japonicum]
MTDRSTAPDSPPTDTWTLANAKARLSEVVDRAQTGPQIITRHGRPNAVVVSADEWARKTARKGTLAEFLLASPLRGADLE
LERVQDEPRDEMP

Nucleotide

• Length: 282 bp

>AT226621 NZ_CP122578:c5207225-5206983 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA