Detailed information of TA system TA226620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2143317..2143888
• Replicon: chromosome
• Accession: NZ_CP088100
• Organism: Bradyrhizobium japonicum strain CC829

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1L3FM63
• Locus tag: BjapCC829_RS09900
• Protein ID: WP_063985200.1
• Coordinates: 2143317..2143670 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8T5V1Z1
• Locus tag: BjapCC829_RS09905
• Protein ID: WP_063985199.1
• Coordinates: 2143667..2143888 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BjapCC829_RS09880 (BjapCC829_09880)	2139608..2139853	+	246	WP_063985204.1	hypothetical protein	-
BjapCC829_RS09885 (BjapCC829_09885)	2140087..2141250	+	1164	WP_063985203.1	crosslink repair DNA glycosylase YcaQ family protein	-
BjapCC829_RS09890 (BjapCC829_09890)	2141375..2141803	-	429	WP_063985202.1	DUF2809 domain-containing protein	-
BjapCC829_RS09895 (BjapCC829_09895)	2141884..2143215	+	1332	WP_063985201.1	MFS transporter	-
BjapCC829_RS09900 (BjapCC829_09900)	2143317..2143670	-	354	WP_063985200.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BjapCC829_RS09905 (BjapCC829_09905)	2143667..2143888	-	222	WP_063985199.1	antitoxin MazE family protein	Antitoxin
BjapCC829_RS09910 (BjapCC829_09910)	2144068..2145174	+	1107	WP_028148596.1	efflux RND transporter periplasmic adaptor subunit	-
BjapCC829_RS09915 (BjapCC829_09915)	2145351..2148488	+	3138	WP_063985198.1	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-107)

Antitoxin

• Antitoxin MazE-like (11-73)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12602.39 Da
• Isoelectric Point: 6.3275

>T226620 WP_063985200.1 NZ_CP088100:c2143670-2143317 [Bradyrhizobium japonicum]
MKRGEVWTVAGGKDYAGKPRPVVIVQDDSFDATNSITVCAFTTDETEAPLFRLKVEPNDGNGLRAPSRLMVDKITTVPKA
KVGTLVGRLDDDDLLRLNRAILVFLGLAGSQRSASAD

Nucleotide

• Length: 354 bp

>T226620 NZ_CP122578:c4735859-4735575 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8284.21 Da
• Isoelectric Point: 7.3202

>AT226620 WP_063985199.1 NZ_CP088100:c2143888-2143667 [Bradyrhizobium japonicum]
MSPSKPKPSRMKVQAHRDRLRAQGLRPIQIWVPDIRSPSFQSEAHRQSLAVATSSHESEDQGFIDAVSDWTDE

Nucleotide

• Length: 222 bp

>AT226620 NZ_CP122578:c4736091-4735849 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A1L3FM63