Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relB-parE/ParE-RHH |
| Location | 296946..297502 | Replicon | plasmid pSU303_3 |
| Accession | NZ_CP088098 | ||
| Organism | Rhizobium leguminosarum bv. viciae strain SU303 | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | A0A198YJZ4 |
| Locus tag | RlegSU303_RS32835 | Protein ID | WP_017958251.1 |
| Coordinates | 296946..297236 (-) | Length | 97 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A7Z0UH60 |
| Locus tag | RlegSU303_RS32840 | Protein ID | WP_017958252.1 |
| Coordinates | 297224..297502 (-) | Length | 93 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RlegSU303_RS32805 (RlegSU303_32805) | 292031..292798 | - | 768 | WP_131709580.1 | 5-oxoprolinase subunit PxpA | - |
| RlegSU303_RS32810 (RlegSU303_32810) | 292836..293831 | - | 996 | WP_231284081.1 | biotin-dependent carboxyltransferase family protein | - |
| RlegSU303_RS32815 (RlegSU303_32815) | 293828..294535 | - | 708 | WP_231284082.1 | 5-oxoprolinase subunit PxpB | - |
| RlegSU303_RS32820 (RlegSU303_32820) | 294532..294957 | - | 426 | WP_131620564.1 | acetyl-CoA carboxylase | - |
| RlegSU303_RS32825 (RlegSU303_32825) | 294954..296339 | - | 1386 | WP_231284083.1 | acetyl-CoA carboxylase biotin carboxylase subunit | - |
| RlegSU303_RS32830 (RlegSU303_32830) | 296339..296782 | - | 444 | WP_017958250.1 | acetyl-CoA carboxylase biotin carboxyl carrier protein subunit | - |
| RlegSU303_RS32835 (RlegSU303_32835) | 296946..297236 | - | 291 | WP_017958251.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| RlegSU303_RS32840 (RlegSU303_32840) | 297224..297502 | - | 279 | WP_017958252.1 | CopG family ribbon-helix-helix protein | Antitoxin |
| RlegSU303_RS32845 (RlegSU303_32845) | 297710..298780 | - | 1071 | WP_231284084.1 | LacI family DNA-binding transcriptional regulator | - |
| RlegSU303_RS32850 (RlegSU303_32850) | 299003..299956 | + | 954 | WP_131709585.1 | sugar phosphate isomerase/epimerase | - |
| RlegSU303_RS32855 (RlegSU303_32855) | 300017..301249 | + | 1233 | WP_231284085.1 | ABC transporter substrate-binding protein | - |
| RlegSU303_RS32860 (RlegSU303_32860) | 301351..302238 | + | 888 | WP_231284086.1 | sugar ABC transporter permease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..333186 | 333186 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 97 a.a. Molecular weight: 11035.72 Da Isoelectric Point: 8.5860
>T226619 WP_017958251.1 NZ_CP088098:c297236-296946 [Rhizobium leguminosarum bv. viciae]
MPQVVFSPAAIRDLERLREFLRLKNPSAAKRAAETILNGLRVLSAHPHIGRLIEDLPEHYREWLIDFGDSGYIARYRIDG
DILTILTVRHQKEAGF
MPQVVFSPAAIRDLERLREFLRLKNPSAAKRAAETILNGLRVLSAHPHIGRLIEDLPEHYREWLIDFGDSGYIARYRIDG
DILTILTVRHQKEAGF
Download Length: 291 bp
>T226619 NZ_CP122578:c4570197-4569664 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
Antitoxin
Download Length: 93 a.a. Molecular weight: 10652.90 Da Isoelectric Point: 5.2246
>AT226619 WP_017958252.1 NZ_CP088098:c297502-297224 [Rhizobium leguminosarum bv. viciae]
MASPTSLKLDDELKGRVQQLAEARRRSSHWIMREAIAQYVEREEKREALRQETLDAWNEFKATGLHVTGAEVEKWLSTWG
TDDELSAPECHK
MASPTSLKLDDELKGRVQQLAEARRRSSHWIMREAIAQYVEREEKREALRQETLDAWNEFKATGLHVTGAEVEKWLSTWG
TDDELSAPECHK
Download Length: 279 bp
>AT226619 NZ_CP122578:c4570474-4570208 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A198YJZ4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7Z0UH60 |