Detailed information of TA system TA226618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 92808..93388
• Replicon: plasmid pSU303_3
• Accession: NZ_CP088098
• Organism: Rhizobium leguminosarum bv. viciae strain SU303

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RlegSU303_RS31870
• Protein ID: WP_094088894.1
• Coordinates: 92808..93191 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0T6ZM79
• Locus tag: RlegSU303_RS31875
• Protein ID: WP_017958375.1
• Coordinates: 93188..93388 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RlegSU303_RS31855 (RlegSU303_31855)	88896..89909	+	1014	WP_017958371.1	alpha-ketoacid dehydrogenase subunit beta	-
RlegSU303_RS31860 (RlegSU303_31860)	89913..91151	+	1239	WP_231284134.1	dihydrolipoamide acetyltransferase family protein	-
RlegSU303_RS31865 (RlegSU303_31865)	91157..92554	+	1398	WP_017958373.1	dihydrolipoyl dehydrogenase	-
RlegSU303_RS31870 (RlegSU303_31870)	92808..93191	-	384	WP_094088894.1	type II toxin-antitoxin system VapC family toxin	Toxin
RlegSU303_RS31875 (RlegSU303_31875)	93188..93388	-	201	WP_017958375.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RlegSU303_RS31880 (RlegSU303_31880)	93773..94699	-	927	WP_231284135.1	LysR substrate-binding domain-containing protein	-
RlegSU303_RS31885 (RlegSU303_31885)	94797..95588	+	792	WP_131719397.1	SDR family oxidoreductase	-
RlegSU303_RS31890 (RlegSU303_31890)	95602..95904	+	303	WP_131620447.1	putative quinol monooxygenase	-
RlegSU303_RS31895 (RlegSU303_31895)	95960..96802	+	843	WP_131719398.1	type 1 glutamine amidotransferase domain-containing protein	-
RlegSU303_RS31900 (RlegSU303_31900)	97154..97987	-	834	WP_094088892.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..333186	333186

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14075.15 Da
• Isoelectric Point: 6.9169

>T226618 WP_094088894.1 NZ_CP088098:c93191-92808 [Rhizobium leguminosarum bv. viciae]
VILADTSIWIDHFRHLDAELRTIIEDDRLLCHPAVAGELALGSLRDRGRVIAFLAAQRQAFVATHDEVMTMIDRHGIFSM
GIGYTDAHLLASIMLDQRAALWTRDKRLQAAAEKAGASLHKPANAHN

Nucleotide

• Length: 384 bp

>T226618 NZ_CP122578:3981599-3981817 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7124.23 Da
• Isoelectric Point: 11.3760

>AT226618 WP_017958375.1 NZ_CP088098:c93388-93188 [Rhizobium leguminosarum bv. viciae]
MRSTINLDDTLMERARSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT226618 NZ_CP122578:3981198-3981572 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0T6ZM79