Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 268890..269566 Replicon plasmid pSU303_1
Accession NZ_CP088096
Organism Rhizobium leguminosarum bv. viciae strain SU303

Toxin (Protein)


Gene name vapC Uniprot ID A0A6N9Z943
Locus tag RlegSU303_RS25835 Protein ID WP_017957377.1
Coordinates 268890..269315 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7V8HB42
Locus tag RlegSU303_RS25840 Protein ID WP_017957376.1
Coordinates 269312..269566 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RlegSU303_RS25815 (RlegSU303_25815) 264231..264890 - 660 WP_131627188.1 sigma-70 family RNA polymerase sigma factor -
RlegSU303_RS25820 (RlegSU303_25820) 265054..265545 + 492 WP_017957380.1 GNAT family N-acetyltransferase -
RlegSU303_RS25825 (RlegSU303_25825) 265628..266410 - 783 WP_131627186.1 siderophore-iron reductase FhuF -
RlegSU303_RS25830 (RlegSU303_25830) 266420..268591 - 2172 WP_131627184.1 TonB-dependent siderophore receptor -
RlegSU303_RS25835 (RlegSU303_25835) 268890..269315 - 426 WP_017957377.1 type II toxin-antitoxin system VapC family toxin Toxin
RlegSU303_RS25840 (RlegSU303_25840) 269312..269566 - 255 WP_017957376.1 plasmid stabilization protein Antitoxin
RlegSU303_RS25845 (RlegSU303_25845) 270219..271504 + 1286 Protein_258 GGDEF domain-containing protein -
RlegSU303_RS25850 (RlegSU303_25850) 271675..272418 + 744 WP_094088036.1 FadR/GntR family transcriptional regulator -
RlegSU303_RS25855 (RlegSU303_25855) 272402..274000 + 1599 WP_131627180.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..938151 938151


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15262.51 Da        Isoelectric Point: 4.8011

>T226616 WP_017957377.1 NZ_CP088096:c269315-268890 [Rhizobium leguminosarum bv. viciae]
MILIDTNVISEPWKPTPDNQVLAWIDAQAVETLFVSAITVAELRFGIAAMPSGRRKTVLYDRLEQEVLPLFSGRVLSFDM
AASQSYSELMARARVAGRAIGKADAYIAATAAANGFIVATRDTSPFEAAGLNVINPWYAPT

Download         Length: 426 bp

>T226616 NZ_CP122578:1911471-1911573 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9218.42 Da        Isoelectric Point: 6.2221

>AT226616 WP_017957376.1 NZ_CP088096:c269566-269312 [Rhizobium leguminosarum bv. viciae]
MAAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNNDVDNLQLVRDKAPAEP
MSFE

Download         Length: 255 bp

>AT226616 NZ_CP122578:c1911580-1911435 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6N9Z943

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8HB42

References