Detailed information of TA system TA226613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 140037..140569
• Replicon: plasmid pWSM1455_4
• Accession: NZ_CP088094
• Organism: Rhizobium laguerreae strain WSM1455

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: RlegWSM1455_RS33555
• Protein ID: WP_077980789.1
• Coordinates: 140037..140330 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: RlegWSM1455_RS33560
• Protein ID: WP_183594594.1
• Coordinates: 140327..140569 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RlegWSM1455_RS33540 (RlegWSM1455_33540)	135884..137854	-	1971	WP_131643139.1	Fe(3+)-hydroxamate ABC transporter permease FhuB	-
RlegWSM1455_RS33545 (RlegWSM1455_33545)	137854..138732	-	879	WP_131643140.1	iron-siderophore ABC transporter substrate-binding protein	-
RlegWSM1455_RS33550 (RlegWSM1455_33550)	138746..139540	-	795	WP_077980788.1	ATP-binding cassette domain-containing protein	-
RlegWSM1455_RS33555 (RlegWSM1455_33555)	140037..140330	-	294	WP_077980789.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RlegWSM1455_RS33560 (RlegWSM1455_33560)	140327..140569	-	243	WP_183594594.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
RlegWSM1455_RS33565 (RlegWSM1455_33565)	140735..141637	-	903	WP_168259205.1	MBL fold metallo-hydrolase	-
RlegWSM1455_RS33570 (RlegWSM1455_33570)	141742..142374	-	633	WP_131641894.1	DsbA family protein	-
RlegWSM1455_RS33575 (RlegWSM1455_33575)	142489..143382	+	894	WP_131641895.1	LysR family transcriptional regulator	-
RlegWSM1455_RS33580 (RlegWSM1455_33580)	143554..143820	+	267	WP_027668792.1	acylphosphatase	-
RlegWSM1455_RS33585 (RlegWSM1455_33585)	143976..144629	+	654	WP_131641896.1	Rieske 2Fe-2S domain-containing protein	-
RlegWSM1455_RS33590 (RlegWSM1455_33590)	144626..145471	+	846	WP_131641897.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..277914	277914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11299.89 Da
• Isoelectric Point: 5.5504

>T226613 WP_077980789.1 NZ_CP088094:c140330-140037 [Rhizobium laguerreae]
MSFKLSAEAEEDIIAIAEQGVRMFGADQAKRYHDELFALFDLIAVNPRIARKRDEIDPPVRIHPFKAHLIVYRIENDETI
FVIRIRHAHEDWASDSI

Nucleotide

• Length: 294 bp

>T226613 NZ_CP122578:732959-733444 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8811.96 Da
• Isoelectric Point: 9.7320

>AT226613 WP_183594594.1 NZ_CP088094:c140569-140327 [Rhizobium laguerreae]
MATMNVSLPGPMKDWVEAQAKTGRYSNASDYVRDLIRRDQTRNDKIAAMQSFVEAGLQSGVGVRSKDELFAEAMARASKS

Nucleotide

• Length: 243 bp

>AT226613 NZ_CP122578:732669-732962 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA