Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 724181..724971 Replicon plasmid pWSM1455_1
Accession NZ_CP088091
Organism Rhizobium laguerreae strain WSM1455

Toxin (Protein)


Gene name TacT2 Uniprot ID -
Locus tag RlegWSM1455_RS27645 Protein ID WP_077976748.1
Coordinates 724474..724971 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID -
Locus tag RlegWSM1455_RS27640 Protein ID WP_077976747.1
Coordinates 724181..724477 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RlegWSM1455_RS27605 (RlegWSM1455_27605) 719236..719427 + 192 WP_206108860.1 hypothetical protein -
RlegWSM1455_RS27610 (RlegWSM1455_27610) 719444..720775 - 1332 WP_131662553.1 epoxide hydrolase -
RlegWSM1455_RS27615 (RlegWSM1455_27615) 721244..721363 - 120 Protein_741 SDR family NAD(P)-dependent oxidoreductase -
RlegWSM1455_RS27620 (RlegWSM1455_27620) 721610..722053 - 444 WP_131661888.1 PIN domain-containing protein -
RlegWSM1455_RS27625 (RlegWSM1455_27625) 722050..722277 - 228 WP_131642915.1 CopG family transcriptional regulator -
RlegWSM1455_RS34310 722295..722420 - 126 WP_260685381.1 hypothetical protein -
RlegWSM1455_RS27630 (RlegWSM1455_27630) 722411..723129 + 719 Protein_745 hypothetical protein -
RlegWSM1455_RS27635 (RlegWSM1455_27635) 723223..723987 + 765 WP_077976746.1 metallophosphoesterase -
RlegWSM1455_RS27640 (RlegWSM1455_27640) 724181..724477 + 297 WP_077976747.1 DUF1778 domain-containing protein Antitoxin
RlegWSM1455_RS27645 (RlegWSM1455_27645) 724474..724971 + 498 WP_077976748.1 GNAT family N-acetyltransferase Toxin
RlegWSM1455_RS27650 (RlegWSM1455_27650) 725419..726477 + 1059 WP_231283901.1 GSU2403 family nucleotidyltransferase fold protein -
RlegWSM1455_RS34315 726620..726895 - 276 Protein_750 ATP-binding protein -
RlegWSM1455_RS27660 (RlegWSM1455_27660) 727125..727445 - 321 Protein_751 Ku protein -
RlegWSM1455_RS27665 (RlegWSM1455_27665) 727634..728296 + 663 WP_231285541.1 transcriptional regulator -
RlegWSM1455_RS27670 (RlegWSM1455_27670) 728428..728988 + 561 WP_077976750.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..1064136 1064136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-142)

Antitoxin

Vibrio phage ICP1, Orf50

(10-97)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 17768.31 Da        Isoelectric Point: 6.9789

>T226610 WP_077976748.1 NZ_CP088091:724474-724971 [Rhizobium laguerreae]
VTLSVPGPLADHHELAEFHSGVPELDDWLRLRARANQASGASRTFVVCEANRVIAYYALASGAVKQSEAPGRFRRNMPDP
IPVAVLGRLAIDQTYQGRGFGRALVRDAGLRLINAAEILGIRGVLVHAISDDARAFYEAVGFLPSPSDPMMLLVGLHDLN
NALTS

Download         Length: 498 bp

>T226610 NZ_CP122574:c156430-155987 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11175.79 Da        Isoelectric Point: 8.5685

>AT226610 WP_077976747.1 NZ_CP088091:724181-724477 [Rhizobium laguerreae]
MPTSHPSKRPDASVALNMRIKPATRNLIDRAAEMLGKTRTDFMLEASERRAEEVLLDRTIFTVSAEVYAEYLARLDAPTQ
PNERLKRTMSTKAPWDEV

Download         Length: 297 bp

>AT226610 NZ_CP122574:c156657-156427 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References