Detailed information of TA system TA226608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 297924..298600
• Replicon: plasmid pWSM1455_1
• Accession: NZ_CP088091
• Organism: Rhizobium laguerreae strain WSM1455

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RlegWSM1455_RS25365
• Protein ID: WP_231285648.1
• Coordinates: 297924..298349 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1S9GB48
• Locus tag: RlegWSM1455_RS25370
• Protein ID: WP_065282799.1
• Coordinates: 298346..298600 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RlegWSM1455_RS25345 (RlegWSM1455_25345)	293264..293923	-	660	WP_231285647.1	sigma-70 family RNA polymerase sigma factor	-
RlegWSM1455_RS25350 (RlegWSM1455_25350)	294087..294578	+	492	WP_077980617.1	GNAT family N-acetyltransferase	-
RlegWSM1455_RS25355 (RlegWSM1455_25355)	294660..295448	-	789	WP_077980616.1	siderophore-iron reductase FhuF	-
RlegWSM1455_RS25360 (RlegWSM1455_25360)	295453..297624	-	2172	WP_168259570.1	TonB-dependent siderophore receptor	-
RlegWSM1455_RS25365 (RlegWSM1455_25365)	297924..298349	-	426	WP_231285648.1	type II toxin-antitoxin system VapC family toxin	Toxin
RlegWSM1455_RS25370 (RlegWSM1455_25370)	298346..298600	-	255	WP_065282799.1	plasmid stabilization protein	Antitoxin
RlegWSM1455_RS25375 (RlegWSM1455_25375)	299319..300536	+	1218	WP_231285649.1	GGDEF domain-containing protein	-
RlegWSM1455_RS25380 (RlegWSM1455_25380)	300711..301454	+	744	WP_231285650.1	FadR/GntR family transcriptional regulator	-
RlegWSM1455_RS25385 (RlegWSM1455_25385)	301438..303036	+	1599	WP_231285651.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1064136	1064136

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15297.56 Da
• Isoelectric Point: 4.5881

>T226608 WP_231285648.1 NZ_CP088091:c298349-297924 [Rhizobium laguerreae]
MILIDTNVISEPWKPTPDYQVLAWIDAQAVETLFVSAITVAELQFGIAAMPSGRRKTVLYDRLEQEVLPLFSGRVLSFDM
AASQSYSELMARARLAGRAIGKADAYIAATAAANGFIVATRDTSPFEAAGLNVINPWYAPT

Nucleotide

• Length: 426 bp

>T226608 NZ_CP122574:c58140-57829 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9192.38 Da
• Isoelectric Point: 6.2221

>AT226608 WP_065282799.1 NZ_CP088091:c298600-298346 [Rhizobium laguerreae]
MAAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNNDVDNLQLVRDKAAAEP
MSFE

Nucleotide

• Length: 255 bp

>AT226608 NZ_CP122574:c57832-57413 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1S9GB48